Rabbit anti-Human, Mouse CYC1 Polyclonal Antibody | anti-CYC1 antibody

CYC1 (Cytochrome C1, Heme Protein, Mitochondrial, Complex III Subunit 4, Complex III Subunit IV, Cytochrome b-c1 Complex Subunit 4, Ubiquinol-cytochrome-c Reductase Complex Cytochrome C1 Subunit, Cytochrome C-1) APC

Cat. Num. AAA6375271

• Gene Names: CYC1; UQCR4
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CYC1; Polyclonal Antibody; CYC1 (Cytochrome C1; Heme Protein; Mitochondrial; Complex III Subunit 4; Complex III Subunit IV; Cytochrome b-c1 Complex Subunit 4; Ubiquinol-cytochrome-c Reductase Complex Cytochrome C1 Subunit; Cytochrome C-1) APC; anti-CYC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYC1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CYC1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human CYC1, aa1-325 (NP_001907.2).
• Immunogen Sequence: MAAAAASLRGVVLGPRGAGLPGARARGLLCSARPGQLPLRTPQAVALSSKSGLSRGRKVMLSALGMLAAGGAGLAVALHSAVSASDLELHPPSYPWSHRGLLSSLDHTSIRRGFQVYKQVCASCHSMDFVAYRHLVGVCYTEDEAKELAAEVEVQDGPNEDGEMFMRPGKLFDYFPKPYPNSEAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIAMAPPIYTDVLEFDDGTPATMSQIAKDVCTFLRWASEPEHDHRKRMGLKMLMMMALLVPLVYTIKRHKWSVLKSRKLAYRPPK
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CYC1 rabbit polyclonal antibody. Western Blot analysis of CYC1 expression in human pancreas.)

Western Blot (WB)

(CYC1 rabbit polyclonal antibody. Western Blot analysis of CYC1 expression in mouse kidney.)

Western Blot (WB)

(CYC1 rabbit polyclonal antibody. Western Blot analysis of CYC1 expression in A-431.)

Western Blot (WB)

(Western Blot analysis of CYC1 expression in transfected 293T cell line by CYC1 polyclonal antibody. Lane 1: CYC1 transfected lysate (35.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-CYC1 antibody

This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.

Product Categories/Family for anti-CYC1 antibody

Antibodies; Cytochrome

References

1. Radiation-induced signaling results in mitochondrial impairment in mouse heart at 4 weeks after exposure to x-rays. Barjaktarovic Z, Schmaltz D, Shyla A, Azimzadeh O, Schulz S, Haagen J, Dorr W, Sarioglu H, Schafer A, Atkinson MJ, Zischka H, Tapio S.PLoS One. 2011;6(12):e27811. Epub 2011 Dec 8.

NCBI and Uniprot Product Information

• NCBI GI #: 21359867
• NCBI GeneID: 1537
• NCBI Accession #: NP_001907.2
• NCBI GenBank Nucleotide #: NM_001916
• UniProt Accession #: P08574; Q5U062; Q6FHS7
• Molecular Weight: 35,422 Da
• NCBI Official Full Name: cytochrome c1, heme protein, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c-1
• NCBI Official Symbol: CYC1
• NCBI Official Synonym Symbols: UQCR4
• NCBI Protein Information: cytochrome c1, heme protein, mitochondrial; complex III subunit 4; complex III subunit IV; cytochrome b-c1 complex subunit 4; ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit
• UniProt Protein Name: Cytochrome c1, heme protein, mitochondrial
• Protein Family: Cytochrome
• UniProt Gene Name: CYC1
• UniProt Synonym Gene Names: Cytochrome c-1
• UniProt Entry Name: CY1_HUMAN

NCBI Description

This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c. Mutations in this gene may cause mitochondrial complex III deficiency, nuclear type 6. [provided by RefSeq, Dec 2013]

Uniprot Description

CYC1: This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain. Belongs to the cytochrome c family.

Protein type: Mitochondrial; Energy Metabolism - oxidative phosphorylation; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; integral to membrane; nucleus

Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; iron ion binding; heme binding

Biological Process: cellular metabolic process; response to glucagon stimulus

Disease: Mitochondrial Complex Iii Deficiency, Nuclear Type 6