Western Blot (WB)
(Western blot analysis of CXCL5 expression in recombinant rat CXCL5 protein 0.5ng (lane 1). CXCL5 at 14KD was detected using rabbit anti- CXCL5 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
anti-Rat CXCL5 Polyclonal Antibody | anti-CXCL5 antibody
Anti-CXCL5 Antibody
Gene Names
Cxcl6; Cxcl5
Reactivity
Rat
Applications
Western Blot, ELISA
Purity
Immunogen Affinity Purified
Synonyms
CXCL5; Polyclonal Antibody; Anti-CXCL5 Antibody; C-X-C motif chemokine 5; AMCFII; chemokine (C-X-C motif) ligand 5; Cxcl5; CXCL5_HUMAN; ENA-78 (8-78); ENA-78(1-78); ENA-78(9-78); ENA78; Epithelial derived neutrophil activating protein 78; Epithelial-derived neutrophil-activating protein 78; Lipopolysaccharide-induced CXC chemokine; Neutrophil activating peptide ENA 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; SCYB5; Small inducible cytokine B5; small inducible cytokine subfamily B (Cys-X-Cys); member 5 (epithelial-derived neutrophil-activating peptide 78); small inducible cytokine subfamily B; member 5; Small-inducible cytokine B5; anti-CXCL5 antibody
Reactivity
Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
130
Applicable Applications for anti-CXCL5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA Concentration: 0.1-0.5ug/ml
Western Blot Concentration: 0.1-0.5ug/ml
Western Blot Concentration: 0.1-0.5ug/ml
Immunogen
E Coli-derived rat CXCL5 recombinant protein (Position: A38-Q130). Rat CXCL5 shares 58.7% and 76.3% amino acid (aa) sequence identity with human and mouse CXCL5, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Western blot analysis of CXCL5 expression in recombinant rat CXCL5 protein 0.5ng (lane 1). CXCL5 at 14KD was detected using rabbit anti- CXCL5 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of CXCL5 expression in recombinant rat CXCL5 protein 0.5ng (lane 1). CXCL5 at 14KD was detected using rabbit anti- CXCL5 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Related Product Information for anti-CXCL5 antibody
Description: Rabbit IgG polyclonal antibody for C-X-C motif chemokine 5(CXCL5) detection. Tested with WB, ELISA in Rat.
Background: C-X-C motif chemokine 5 or ENA-78 is a protein that in humans is encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed ineosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on human chromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodelling. Also, CXCL5 has been described to regulate neutrophil homeostasis.
Background: C-X-C motif chemokine 5 or ENA-78 is a protein that in humans is encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed ineosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on human chromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodelling. Also, CXCL5 has been described to regulate neutrophil homeostasis.
References
1. "Entrez Gene: CXCL5 chemokine (C-X-C motif) ligand 5". 2. Chang MS, McNinch J, Basu R, Simonet S (Nov 1994). "Cloning and characterization of the human neutrophil-activating peptide (ENA-78) gene". J Biol Chem 269 (41): 25277-82. 3. Persson T, Monsef N, Andersson P, Bjartell A, Malm J, Calafat J, Egesten A (2003). "Expression of the neutrophil-activating CXC chemokine ENA-78/CXCL5 by human eosinophils". Clin. Exp. Allergy 33 (4): 531-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,263 Da
NCBI Official Full Name
C-X-C motif chemokine 5
NCBI Official Synonym Full Names
C-X-C motif chemokine ligand 6
NCBI Official Symbol
Cxcl6
NCBI Official Synonym Symbols
Cxcl5
NCBI Protein Information
C-X-C motif chemokine 5
UniProt Protein Name
C-X-C motif chemokine 5
Protein Family
UniProt Gene Name
Cxcl5
UniProt Synonym Gene Names
Scyb5
UniProt Entry Name
CXCL5_RAT
NCBI Description
mouse homolog is a member of the CXC chemokine family, is a neutrophil chemoattractant and is rapidly induced in response to muscle injury [RGD, Feb 2006]
Uniprot Description
CXCL6: Chemotactic for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis
Cellular Component: extracellular space
Molecular Function: chemokine activity; CXCR chemokine receptor binding
Biological Process: cytokine production; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; leukocyte homeostasis; midbrain development; neutrophil mediated immunity; organ regeneration; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; regulation of cell proliferation; regulation of chemokine production; response to gamma radiation; response to lipopolysaccharide
Research Articles on CXCL5
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Product Notes
The CXCL5 cxcl5 (Catalog #AAA177726) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CXCL5 Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CXCL5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA Concentration: 0.1-0.5ug/ml Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the CXCL5 cxcl5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCL5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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