Mouse anti-Human VE-Cadherin Monoclonal Antibody | anti-CDH5 antibody

VE-Cadherin (Extracellular region) Antibody

Cat. Num. AAA474234

• Gene Names: CDH5; 7B4; CD144
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunocytochemistry
• Purity: Protein A chromatography
• Synonyms: VE-Cadherin; Monoclonal Antibody; VE-Cadherin (Extracellular region) Antibody; VE-Cadherin (Extracellular region); Cadherin VE M035; anti-CDH5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M035
• Specificity: Clone M035 detects a 140kDa* band corresponding to VE-cadherin in western blots of human endothelial cells. The antibody is recommended for use in western blot, ELISA and immunocytochemsitry, as well as for function blocking VE-cadherin homotyic adhesion.
• Purity/Purification: Protein A chromatography
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-CDH5 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), FB
• Application Notes: ELISA: 1:1000; FB: 1:25; ICC: 1:100; WB: 1:250
• Immunogen: Clone M035 was generated from a recombinant protein that included amino acids in the extracellular region of human VE-Cadherin. This sequence is not conserved in other cadherin family members.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot image of humanuMbilical vein endothelial cells stimulated with pervanadate (1mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of VE-Cadherin in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-VE-Cadherin (CM0351). The antibody was detected using goat anti-mouse DyLight 594.)

Related Product Information for anti-CDH5 antibody

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with beta- or gamma-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.

References

Wallez Y. et al. (2007) Oncogene 26:1067.
Baumeister U. et al. (2005) EMBOJ 24:1686.
Potter M.D. et al. (2005) J Biol. Chem. 280(36):31906

NCBI and Uniprot Product Information

• NCBI GI #: 166362713
• NCBI GeneID: 1003
• NCBI Accession #: NP_001786.2
• NCBI GenBank Nucleotide #: NM_001795.3
• UniProt Accession #: P33151
• Molecular Weight: 87,528 Da
• NCBI Official Full Name: cadherin-5 preproprotein
• NCBI Official Synonym Full Names: cadherin 5, type 2 (vascular endothelium)
• NCBI Official Symbol: CDH5
• NCBI Official Synonym Symbols: 7B4; CD144
• NCBI Protein Information: cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium)
• UniProt Protein Name: Cadherin-5
• Protein Family: Cadherin
• UniProt Gene Name: CDH5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: adherens junction; tight junction; membrane; plasma membrane; integral to membrane; intercellular junction; cell junction

Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding

Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; homophilic cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood vessel maturation