Western Blot (WB)
((0.3ug/ml) staining of Human Colorectal cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CX3CL1/fractalkine Polyclonal Antibody | anti-CX3CL1 antibody
Goat anti-CX3CL1/fractalkine (aa214-226) Antibody
Western Blot: Approx 45kDa band observed in Human Colorectal cancer lysates (calculated MW of 42.2kDa according to NP_002987.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Colorectal cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Colorectal cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16q13
Cellular Component: cell projection; cell surface; extracellular region; extracellular space; integral to membrane; perinuclear region of cytoplasm; plasma membrane
Molecular Function: CCR chemokine receptor binding; chemokine activity; CX3C chemokine receptor binding; integrin binding; protein binding; receptor binding
Biological Process: aging; angiogenesis involved in wound healing; cell adhesion; chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; G-protein coupled receptor protein signaling pathway; immune response; integrin activation; leukocyte adhesive activation; leukocyte chemotaxis; leukocyte migration during inflammatory response; lymphocyte chemotaxis; macrophage chemotaxis; microglial cell activation; monocyte chemotaxis; negative regulation of cytokine secretion; negative regulation of vasoconstriction; neutrophil chemotaxis; platelet activation; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; positive regulation of GTPase activity; positive regulation of inflammatory response; positive regulation of neuroblast proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of transforming growth factor-beta1 production; regulation of inhibitory postsynaptic membrane potential; response to hypoxia
Research Articles on CX3CL1
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Product Notes
The CX3CL1 cx3cl1 (Catalog #AAA423425) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CX3CL1/fractalkine (aa214-226) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's CX3CL1/fractalkine can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 45kDa band observed in Human Colorectal cancer lysates (calculated MW of 42.2kDa according to NP_002987.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the CX3CL1 cx3cl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KTSEAPSTQD PST. It is sometimes possible for the material contained within the vial of "CX3CL1/fractalkine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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