Rabbit anti-Human CTSB / Cathepsin B Polyclonal Antibody | anti-CTSB antibody

Rabbit Polyclonal to Human CTSB / Cathepsin B

Cat. Num. AAA248791

• Gene Names: CTSB; APPS; CPSB
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Immunoaffinity Purified
• Synonyms: CTSB / Cathepsin B; Polyclonal Antibody; Rabbit Polyclonal to Human CTSB / Cathepsin B; Anti-CTSB / Cathepsin B Antibody (Internal) IHC-plus; CTSB; Cathepsin B1; Cathepsin B; Cathepsins B; CPSB; Cysteine protease; Preprocathepsin B; APP secretase; APPS; Human CTSB; anti-CTSB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS with 0.09% (w/v) sodium azide
• Sequence Length: 339

• Applicable Applications for anti-CTSB antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:10 - 1:50), IHC-P (10 ug/ml), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH conjugated synthetic peptide selected from the Center region of human CATB.
• Immunogen Type: Synthetic peptide
• Immunogen: CTSB / Cathepsin B antibody was raised against kLH conjugated synthetic peptide selected from the Center region of human CATB.
• Antigen Modification: Internal
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-CTSB / Cathepsin B antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CATB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HepG2 cells using CATB Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot of CATB Antibody (Center) in Y79 cell line lysates (35 ug/lane). CATB (arrow) was detected using the purified Pab.)

Related Product Information for anti-CTSB antibody

Cathepsin B is a lysosomal cysteine proteinase synthesized as a zymogen of 39-47 kilodaltons (kD), which is subsequently converted into an active single- chain form of 33 kD (CB33) and, by additional processing, into the active 2-chain form containing a heavy chain of 27-29 kD (CB(27-29)) and a light chain of 4-6 kD.

Product Categories/Family for anti-CTSB antibody

Family: Protease; Subfamily: Cysteine C1

NCBI and Uniprot Product Information

• NCBI GI #: 4503139
• NCBI GeneID: 1508
• NCBI Accession #: NP_001899.1
• NCBI GenBank Nucleotide #: NM_001908.3
• UniProt Accession #: P07858; Q503A6; Q96D87; B3KQR5; B3KRR5
• Molecular Weight: 37,822 Da
• NCBI Official Full Name: cathepsin B preproprotein
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: CTSB
• NCBI Official Synonym Symbols: APPS; CPSB
• NCBI Protein Information: cathepsin B; cathepsin B1; APP secretase; cysteine protease; amyloid precursor protein secretase
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: CTSB
• UniProt Synonym Gene Names: CPSB; APPS
• UniProt Entry Name: CATB_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.

Protein type: Motility/polarity/chemotaxis; Protease; EC 3.4.22.1; Autophagy

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular space; mitochondrion; intracellular membrane-bound organelle; perinuclear region of cytoplasm; lysosome; nucleolus; melanosome; extracellular region; intracellular

Molecular Function: collagen binding; peptidase activity; protein binding; proteoglycan binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; proteolysis involved in cellular protein catabolic process; epithelial cell differentiation; regulation of catalytic activity; toll-like receptor signaling pathway; innate immune response; decidualization; proteolysis