Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using BCR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Rabbit Bcr Polyclonal Antibody | anti-BCR antibody
Bcr Rabbit pab Antibody
Gene Names
BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity purification
Synonyms
Bcr; Polyclonal Antibody; Bcr Rabbit pab Antibody; BCR; Bcr ; ALL; BCR1; CML; D22S11; D22S662; FLJ16453; PHL; anti-BCR antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
1271
Applicable Applications for anti-BCR antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Western Blot (WB): 1/1000-2000
Immunohistochemistry (IHC): 1/50
Immunocytochemistry (ICC): 1/100
Immunoprecipitation (IP): 1/30
Flow Cytometry (FC/FACS): 1/20
Immunohistochemistry (IHC): 1/50
Immunocytochemistry (ICC): 1/100
Immunoprecipitation (IP): 1/30
Flow Cytometry (FC/FACS): 1/20
Immunogen
A synthetic -peptide of human Bcr
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using BCR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using BCR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using BCR antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using BCR antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using BCR antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using BCR antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-BCR antibody
Rabbit polyclonal to Bcr
The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1). The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region may be involved in a GTPase activating function for the small GTP-binding protein Rac (2,3). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator (4). The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5). Tyr177 of Bcr is phosphorylated in the Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl (6). Phosphorylated Tyr177 of Bcr provides a docking site for Gab2 and GRB2 (7,8).
The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1). The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region may be involved in a GTPase activating function for the small GTP-binding protein Rac (2,3). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator (4). The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5). Tyr177 of Bcr is phosphorylated in the Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl (6). Phosphorylated Tyr177 of Bcr provides a docking site for Gab2 and GRB2 (7,8).
References
1. Groffen, J. et al. (1984) Cell 36, 93-99. 2. Maru, Y. et al. (1991) Cell 67, 459-468. 3. Che, W. et al. (2001) Circulation 104, 1399-1406. 4. Abe, J. I. et al. (2001) Ann. N.Y. Acad. Sci. 947, 341-343. 5. Voncken, J. W. et al. (1995) Cell 80, 719-728. 6. He, Y. et al. (2002) Blood 99, 2957-2968. 7. Sattler, M. et al. (2002) Cancer Cell 1, 479-492. 8. Warmuth, M. et al. (1995) J. Biol. Chem. 272, 33260-33270.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
1271
NCBI Official Full Name
breakpoint cluster region protein isoform 1
NCBI Official Synonym Full Names
breakpoint cluster region
NCBI Official Symbol
BCR
NCBI Official Synonym Symbols
ALL; CML; PHL; BCR1; D22S11; D22S662
NCBI Protein Information
breakpoint cluster region protein; BCR/FGFR1 chimera protein; FGFR1/BCR chimera protein; renal carcinoma antigen NY-REN-26
UniProt Protein Name
Breakpoint cluster region protein
Protein Family
UniProt Gene Name
BCR
UniProt Synonym Gene Names
BCR1; D22S11
UniProt Entry Name
BCR_HUMAN
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Product Notes
The BCR bcr (Catalog #AAA125290) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bcr Rabbit pab Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bcr can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Western Blot (WB): 1/1000-2000 Immunohistochemistry (IHC): 1/50 Immunocytochemistry (ICC): 1/100 Immunoprecipitation (IP): 1/30 Flow Cytometry (FC/FACS): 1/20. Researchers should empirically determine the suitability of the BCR bcr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcr, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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