Rabbit anti-Human, Mouse CTCF Polyclonal Antibody | anti-CTCF antibody

Rabbit Polyclonal (IgG) to Human CTCF

Cat. Num. AAA248369

• Gene Names: CTCF; MRD21
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CTCF; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human CTCF; Anti-CTCF Antibody (C-Terminus) IHC-plus; 11-zinc finger protein; CCCTC-binding factor; Transcriptional repressor CTCF; CTCFL paralog; Human CTCF; anti-CTCF antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody reacts with endogenous CTCF protein. A BLAST analysis was used to suggest reactivity with CTCF from human, mouse, horse, bovine, panda, rabbit, Danio, and chicken based on a 100% homology with the immunizing sequence. Cross-reactivity with CTCF from other sources has not been determined.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
• Sequence Length: 727

• Applicable Applications for anti-CTCF antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000 - 1:50000), IHC-P (5 ug/ml), WB (1:1000); Usage: This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 82.8 kD or 150 kD in size corresponding to CTCF by western blotting in the...
• Target Species: Human
• Immunogen Description: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.
• Immunogen Type: Synthetic peptide
• Immunogen: CTCF antibody was raised against this affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-CTCF antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of Rabbit Anti-CTCF antibody. Lane 1: H929 nuclear cell lysates. Load: 30 ug per lane. Primary antibody: CTCF antibody at 1.2 g/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:5000 for 45 min at RT. Block: 3% BSA overnight at 4C. Predicted/Observed size: 82.8 kDa or 150 kDa for CTCF. Other band(s): CTCF splice variants.)

Product Categories/Family for anti-CTCF antibody

Family: Transcription factor

NCBI and Uniprot Product Information

• NCBI GI #: 5729790
• NCBI GeneID: 10664
• NCBI Accession #: NP_006556.1
• NCBI GenBank Nucleotide #: NM_006565.3
• UniProt Accession #: P49711; Q53XI7; Q59EL8; B5MC38
• Molecular Weight: 45,998 Da
• NCBI Official Full Name: transcriptional repressor CTCF isoform 1
• NCBI Official Synonym Full Names: CCCTC-binding factor (zinc finger protein)
• NCBI Official Symbol: CTCF
• NCBI Official Synonym Symbols: MRD21
• NCBI Protein Information: transcriptional repressor CTCF; CTCFL paralog; 11-zinc finger protein; 11 zinc finger transcriptional repressor
• UniProt Protein Name: Transcriptional repressor CTCF
• Protein Family: Transcriptional repressor
• UniProt Gene Name: CTCF
• UniProt Entry Name: CTCF_HUMAN

NCBI Description

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

CTCF: a widely expressed, sequence-specific DNA-binding transcriptional regulator, insulator, and organizer of higher-order chromatin structure. Acts as a tumor suppressor. Contains 11 C2H2-type zinc fingers. Involved in promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mediates pairing between X chromosomes and interactions between distant regulatory elements. Binds to promoters of c-myc, PLK, PIM1 and APP. A critical regulator of cell-cycle arrest and death after B cell receptor signaling in immature B cells. CTCF, together with YY1 and Tsix, form a regulated epigenetic switch for X-inactivation.

Protein type: C2H2-type zinc finger protein; Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 16q21-q22.3

Cellular Component: nucleoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein binding; zinc ion binding; chromatin insulator sequence binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: genetic imprinting; maintenance of DNA methylation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of histone methylation; regulation of histone acetylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; nucleosome positioning; chromosome segregation; regulation of molecular function, epigenetic; DNA methylation; negative regulation of transcription, DNA-dependent

Disease: Mental Retardation, Autosomal Dominant 21