Rabbit anti-Human CTBP Polyclonal Antibody | anti-CTBP antibody

CTBP antibody (pAb)

Cat. Num. AAA388605

• Gene Names: CTBP1; BARS
• Reactivity: Human
• Applications: Western Blot
• Synonyms: CTBP; Polyclonal Antibody; CTBP antibody (pAb); CTBP1; BARS; anti-CTBP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Form/Format: Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

• Applicable Applications for anti-CTBP antibody: Western Blot (WB)
• Application Notes: Applications Validated:; WB: 1:500-1:2,000 dilution; Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
• Immunogen: This CTBP antibody was raised against a peptide within the C-terminal region of human CTBP.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(CTBP antibody (pAb) tested by Western blot. Nuclear extract of Jurkat cells (30 ug per lane) probed with CTBP antibody (pAb) at a dilution of 1:2,000.)

Related Product Information for anti-CTBP antibody

Short Description: Our CTBP antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.

Background: CTBP (C-terminal Binding Protein, CTBP1) is a transcriptional repressor that was originally identified by virtue of its ability to bind the C-terminus of E1A proteins. CTBP serves as a co-repressor for a number of DNA binding transcription factors. CTBP is part of a variety of co-repressor complexes, many of which include HDAC proteins. CTBP also interacts with vertebrate Polycomb proteins including Pc2, suggesting that repression of many CTBP-regulated genes also involves recruitment of the Polycomb complex.

Product Categories/Family for anti-CTBP antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4557497
• NCBI GeneID: 1487
• NCBI Accession #: NP_001319; AB_2793366
• NCBI GenBank Nucleotide #: NM_001328.2
• UniProt Accession #: Q13363; Q4W5N3; Q7Z2Q5
• Molecular Weight: 46,405 Da
• NCBI Official Full Name: C-terminal-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: C-terminal binding protein 1
• NCBI Official Symbol: CTBP1
• NCBI Official Synonym Symbols: BARS
• NCBI Protein Information: C-terminal-binding protein 1
• UniProt Protein Name: C-terminal-binding protein 1
• Protein Family: C-terminal-binding protein
• UniProt Gene Name: CTBP1
• UniProt Synonym Gene Names: CTBP; CtBP1
• UniProt Entry Name: CTBP1_HUMAN

NCBI Description

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

CTBP1: Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity. Homo- or heterodimer. Heterodimer with CTBP2. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)- specific genes expression. Interacts with GLIS2, FOXP2, HDAC4, HDAC5, HDAC9 and ZNF217. Interacts with adenovirus E1A protein (via its C-terminus); the interaction disrupts the interaction of CTBP1 with RBBP8. Interacts with Epstein-Barr virus EBNA3 and EBNA6. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif); the interaction is disrupted by binding to adenovirus E1A. Interacts with FOXP1, HIPK2, PNN, NRIP1, MECOM, ZNF366, ZFHX1B and WIZ. Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.-; Oxidoreductase; Cell cycle regulation; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; protein homodimerization activity; transcription factor activity; NAD binding; transcription factor binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: negative regulation of cell proliferation; positive regulation of histone deacetylation; transcription, DNA-dependent; regulation of cell cycle; white fat cell differentiation; negative regulation of transcription from RNA polymerase II promoter; viral genome replication; negative regulation of transcription, DNA-dependent; Golgi organization and biogenesis; protein amino acid phosphorylation; negative regulation of histone acetylation

Disease: Wolf-hirschhorn Syndrome