Rabbit anti-Human CSTF2 Polyclonal Antibody | anti-CSTF2 antibody

CSTF2 (Cleavage Stimulation Factor 64kD Subunit, CSTF 64kD Subunit, CF-1 64kD Subunit, CstF-64,Cleavage Stimulation Factor 2) (AP)

Cat. Num. AAA6374940

• Gene Names: CSTF2; CstF-64
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CSTF2; Polyclonal Antibody; CSTF2 (Cleavage Stimulation Factor 64kD Subunit; CSTF 64kD Subunit; CF-1 64kD Subunit; CstF-64; Cleavage Stimulation Factor 2) (AP); anti-CSTF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CSTF2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CSTF2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human CSTF2, aa1-577 (NP_001316.1).
• Immunogen Sequence: MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQMFELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHGAGPGSGSNVSMNQQNPQAPQAQSLGGMHVNGAPPLMQASMQGGVPAPGQMPAAVTGPGPGSLAPGGGMQAQVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPPHQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLDGRGGRDPRGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQVPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAP
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CSTF2 expression in transfected 293T cell line by CSTF2 polyclonal antibody. Lane 1: CSTF2 transfected lysate (61kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-CSTF2 antibody

CSTF2 is one of three (including CSTF1 and CSTF3) cleavage stimulation factors which combine to form CSTF which is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. CSTF2 contains a ribonucleoprotein-type RNA binding domain. CSTF2 is upregulated during activation of B cells which results in the switch of IgM heavy chain mRNA from membrane bound form to the secreted form.

Product Categories/Family for anti-CSTF2 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4557493
• NCBI GeneID: 1478
• NCBI Accession #: NP_001316.1
• NCBI GenBank Nucleotide #: NM_001325
• UniProt Accession #: P33240; Q5H951; Q6LA74; Q8N502
• Molecular Weight: 60,959 Da
• NCBI Official Full Name: cleavage stimulation factor subunit 2
• NCBI Official Synonym Full Names: cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
• NCBI Official Symbol: CSTF2
• NCBI Official Synonym Symbols: CstF-64
• NCBI Protein Information: cleavage stimulation factor subunit 2; CF-1 64 kDa subunit; CSTF 64 kDa subunit; betaCstF-64 variant 2; cleavage stimulation factor 64 kDa subunit
• UniProt Protein Name: Cleavage stimulation factor subunit 2
• Protein Family: Cleavage stimulation factor
• UniProt Gene Name: CSTF2
• UniProt Synonym Gene Names: CSTF 64 kDa subunit; CstF-64
• UniProt Entry Name: CSTF2_HUMAN

NCBI Description

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq, Jul 2008]

Uniprot Description

CSTF2: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: Xq22.1

Cellular Component: nucleoplasm; mRNA cleavage and polyadenylation specificity factor complex; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; RNA splicing; mRNA polyadenylation; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; mRNA cleavage