Western Blot (WB)
(Western blot analysis of extracts from Mouse cancer, using CSTF1 Antibody. Lane 1 was treated with the blocking peptide.)
Rabbit CSTF1 Polyclonal Antibody | anti-CSTF1 antibody
CSTF1 Antibody
Gene Names
CSTF1; CstF-50; CstFp50
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CSTF1; Polyclonal Antibody; CSTF1 Antibody; CF 1 50 kDa subunit; CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; Cleavage stimulation factor 50kDa subunit; Cleavage stimulation factor; Cleavage stimulation factor subunit 1; 3 prime pre RNA; subunit 1; 50 kD; cleavage stimulation factor; 3' pre RNA; 50kD; 50kDa; CstF 50; CSTF 50 kDa subunit; CstF-50; CSTF1_HUMAN; CstFp50; OTTHUMP00000031332; anti-CSTF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CSTF1 antibody detects endogenous levels of total CSTF1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
431
Applicable Applications for anti-CSTF1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Bovine (100%), Horse (91%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Mouse cancer, using CSTF1 Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse cancer, using CSTF1 Antibody. Lane 1 was treated with the blocking peptide.)
Immunofluorescene (IF)
(MBS9604703 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9604703 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells using CSTF1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells using CSTF1 antibody.)
Related Product Information for anti-CSTF1 antibody
Function: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.
Subunit Structure: Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). Interacts directly with CSTF3. Interacts with BARD1.
Post-translational Modifications: The N-terminus is blocked.
Similarity: The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3.N-terminus mediates homodimerization.
Subunit Structure: Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). Interacts directly with CSTF3. Interacts with BARD1.
Post-translational Modifications: The N-terminus is blocked.
Similarity: The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3.N-terminus mediates homodimerization.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 48 kD
Predicted: 49 kDa
Predicted: 49 kDa
NCBI Official Full Name
cleavage stimulation factor subunit 1
NCBI Official Synonym Full Names
cleavage stimulation factor subunit 1
NCBI Official Symbol
CSTF1
NCBI Official Synonym Symbols
CstF-50; CstFp50
NCBI Protein Information
cleavage stimulation factor subunit 1
UniProt Protein Name
Cleavage stimulation factor subunit 1
Protein Family
UniProt Gene Name
CSTF1
UniProt Synonym Gene Names
CSTF 50 kDa subunit; CstF-50
NCBI Description
This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.
Research Articles on CSTF1
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Product Notes
The CSTF1 cstf1 (Catalog #AAA9604703) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CSTF1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CSTF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CSTF1 cstf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSTF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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