Rabbit anti-Human CSTA Polyclonal Antibody | anti-CSTA antibody

CSTA, CT (CSTA, STF1, STFA, Cystatin-A, Cystatin-AS, Stefin-A) (AP)

Cat. Num. AAA6289506

• Gene Names: CSTA; AREI; STF1; STFA
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CSTA; Polyclonal Antibody; CT (CSTA; STF1; STFA; Cystatin-A; Cystatin-AS; Stefin-A) (AP); Stefin-A; anti-CSTA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CSTA antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CSTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-98 amino acids from the C-terminal region of human CSTA.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CSTA Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CSTA antibody detected the CSTA protein (arrow).)

Western Blot (WB)

(Western blot analysis of CSTA (arrow) using rabbit polyclonal CSTA Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CSTA gene.)

Immunohistochemistry (IHC)

(CSTA Antibody (C-term) (AP14350b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CSTA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CSTA antibody

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and test tools for cancer.

Product Categories/Family for anti-CSTA antibody

Antibodies; Protease Inhibitors

References

O'Seaghdha, C.M., et al. Hum. Mol. Genet. 19(21):4296-4303(2010). Kestenbaum, B., et al. J. Am. Soc. Nephrol. 21(7):1223-1232(2010). Takai, T., et al. J. Immunol. 183(12):7958-7965(2009). Vasilopoulos, Y., et al. Eur. J. Hum. Genet. 16(8):1002-1009(2008). Parker, B.S., et al. J. Pathol. 214(3):337-346(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4885165
• NCBI GeneID: 1475
• NCBI Accession #: NP_005204.1
• NCBI GenBank Nucleotide #: NM_005213.3
• UniProt Accession #: P01040
• Molecular Weight: 11,006 Da
• NCBI Official Full Name: cystatin-A
• NCBI Official Synonym Full Names: cystatin A (stefin A)
• NCBI Official Symbol: CSTA
• NCBI Official Synonym Symbols: AREI; STF1; STFA
• NCBI Protein Information: cystatin-A; cystatin AS
• UniProt Protein Name: Cystatin-A
• Protein Family: Cystatin
• UniProt Gene Name: CSTA
• UniProt Synonym Gene Names: STF1; STFA
• UniProt Entry Name: CYTA_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

CSTA: This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. Defects in CSTA are the cause of ichthyosis exfoliative autosomal recessive ichthyosis bullosa of Siemens-like (AREI). A form of congenital exfoliative ichthyosis, sharing some features with ichthyosis bullosa of Siemens and annular epidermolytic ichthyosis. AREI presents shortly after birth as dry, scaly skin over most of the body with coarse peeling of non- erythematous skin on the palms and soles, which is exacerbated by excessive moisture and minor trauma. Electron microscopy analysis of skin biopsies, reveals mostly normal-appearing upper layers of the epidermis, but prominent intercellular edema of the basal and suprabasal cell layers with aggregates of tonofilaments in the basal keratinocytes. Belongs to the cystatin family.

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cornified envelope; nucleus

Molecular Function: protein binding, bridging; protease binding; cysteine protease inhibitor activity; structural molecule activity

Biological Process: negative regulation of proteolysis; keratinocyte differentiation; cell-cell adhesion; negative regulation of peptidase activity; peptide cross-linking

Disease: Exfoliative Ichthyosis, Autosomal Recessive, Ichthyosis Bullosa Of Siemens-like