Rabbit anti-Human CSNK1G3 Polyclonal Antibody | anti-CSNK1G3 antibody

CSNK1G3, CT (Casein Kinase I Isoform gamma-3, CKI-gamma 3)

Cat. Num. AAA625846

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CSNK1G3; Polyclonal Antibody; CT (Casein Kinase I Isoform gamma-3; CKI-gamma 3); Anti -CSNK1G3; anti-CSNK1G3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CK1g3.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CSNK1G3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human CK1g3 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CK1g3 Pab in placenta lysate. CK1g3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-CSNK1G3 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The casein kinase 1 (CK1) group consists of 12 kinases including CK1, TTBK (tau tubulin kinase), and VRK (vaccinia-related kinase) families The receptor guanylate cyclase (RGC) group consists of 5 kinases similar in domain sequence to TKs (ANP, CYG).

Product Categories/Family for anti-CSNK1G3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 113462010
• NCBI GeneID: 1456
• NCBI Accession #: NP_001038188.1
• NCBI GenBank Nucleotide #: NM_001044723.1
• UniProt Accession #: Q9Y6M4; Q86WZ7; Q9Y6M3
• Molecular Weight: 51,389 Da
• NCBI Official Full Name: casein kinase I isoform gamma-3 isoform 4
• NCBI Official Synonym Full Names: casein kinase 1, gamma 3
• NCBI Official Symbol: CSNK1G3
• NCBI Protein Information: casein kinase I isoform gamma-3; CKI-gamma 3; OTTHUMP00000159174; OTTHUMP00000159175; OTTHUMP00000223000; OTTHUMP00000223001; OTTHUMP00000223002; OTTHUMP00000227071
• UniProt Protein Name: Casein kinase I isoform gamma-3
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1G3
• UniProt Entry Name: KC1G3_HUMAN

NCBI Description

This gene encodes a member of a family of serine/threonine protein kinases that phosphorylate caseins and other acidic proteins. A related protein in the African clawed frog participates in the transmission of Wnt/beta-catenin signaling. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

CK1G3: Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate. Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CK1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 5q23

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of cell shape; peptidyl-serine phosphorylation; Wnt receptor signaling pathway; protein modification process; endocytosis; signal transduction