Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.kidney section using CYS1 Antibody (C-term). MBS9208031 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Rabbit anti-Human, mouse CYS1 Polyclonal Antibody | anti-CYS1 antibody
CYS1 Antibody (C-term)
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CYS1; Polyclonal Antibody; CYS1 Antibody (C-term); Cystin-1; Cilia-associated protein; anti-CYS1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CYS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 142-177 amino acids from the C-terminal region of human CYS1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
158
Applicable Applications for anti-CYS1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
IHC~~1:25
WB~~1:1000
WB~~1:1000
Cellular Location
Cell projection, cilium membrane; Lipid-anchor. Cytoplasm, cytoskeleton, cilium axoneme. Note=Expression is enriched in the ciliary axoneme. Localization to cilium is mediated via interaction with UNC119 and UNC119B, which bind to the myristoyl moiety of the N- terminus
Tissue Location
Expressed at high levels in the kidney and pancreas. Moderate expression seen in the skeletal muscle, liver and heart. A weak expression seen in the brain, lung, uterus, prostate, testis, small intestine and colon
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks.
For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.kidney section using CYS1 Antibody (C-term). MBS9208031 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.kidney section using CYS1 Antibody (C-term). MBS9208031 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Western Blot (WB)
(Western blot analysis of lysates from human heart, human kidney and mouse kidney tissue lysate(from left to right), using CYS1 Antibody (C-term). MBS9208031 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from human heart, human kidney and mouse kidney tissue lysate(from left to right), using CYS1 Antibody (C-term). MBS9208031 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Product Categories/Family for anti-CYS1 antibody
References
Fliegauf M.,et al.Pediatr. Nephrol. 18:498-505(2003).
Hou X.,et al.J. Clin. Invest. 109:533-540(2002).
Wright K.J.,et al.Genes Dev. 25:2347-2360(2011).
Hou X.,et al.J. Clin. Invest. 109:533-540(2002).
Wright K.J.,et al.Genes Dev. 25:2347-2360(2011).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: 16393
NCBI Official Full Name
cystin-1
NCBI Official Synonym Full Names
cystin 1
NCBI Official Symbol
CYS1
NCBI Protein Information
cystin-1
UniProt Protein Name
Cystin-1
Protein Family
UniProt Gene Name
CYS1
UniProt Entry Name
CYS1_HUMAN
Uniprot Description
CYS1: Interacts (when myristoylated) with UNC119 and UNC119B; interaction is required for localization to cilium
Chromosomal Location of Human Ortholog: 2p25.1
Cellular Component: axoneme; cytosol; lipid raft
Molecular Function: protein binding
Biological Process: organelle organization and biogenesis; kidney development; inner ear development
Research Articles on CYS1
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Product Notes
The CYS1 cys1 (Catalog #AAA9208031) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYS1 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CYS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). IHC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the CYS1 cys1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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