Goat CSNK1E / CK1 Epsilon Polyclonal Antibody | anti-CSNK1E antibody

Goat Polyclonal to Human CSNK1E / CK1 Epsilon

Cat. Num. AAA242034

• Gene Names: CSNK1E; HCKIE; CKIepsilon
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Mouse, Rat, Bat, Dog, Horse, Pig, Chicken (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CSNK1E / CK1 Epsilon; Polyclonal Antibody; Goat Polyclonal to Human CSNK1E / CK1 Epsilon; Anti-CSNK1E / CK1 Epsilon Antibody (C-Terminus) IHC-plus; CSNK1E; Casein kinase 1 epsilon; Casein kinase i epsilon; CK1 epsilon; CKIE; CKIepsilon; CKI-epsilon; HCKIE; Casein kinase 1; epsilon; Human CSNK1E; CK1 Epsilon; anti-CSNK1E antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Mouse, Rat, Bat, Dog, Horse, Pig, Chicken (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CSNK1E. Both reported variants represent identical protein (NP_001885.1 and NP_689407.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 416

• Applicable Applications for anti-CSNK1E antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (5 ug/ml), WB (0.3 - 1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-PASQTSVPFDHLGK from the C-terminus of human CSNK1E (NP_001885.1; NP_689407.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: CSNK1E / CK1 Epsilon antibody was raised against synthetic peptide C-PASQTSVPFDHLGK from the C-terminus of human CSNK1E (NP_001885.1; NP_689407.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Turkey, Chicken, Platypus (93%); Xenopus (86%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CSNK1E antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (1 ug/ml) staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-CSNK1E antibody

Casein Kinase 1, Epsilon belongs to the casein kinase subfamily.

Product Categories/Family for anti-CSNK1E antibody

Family: Protein Kinase; Subfamily: Casein kinase I

NCBI and Uniprot Product Information

• NCBI GI #: 4503093
• NCBI GeneID: 1454
• NCBI Accession #: NP_001885.1
• NCBI GenBank Nucleotide #: NM_001894.4
• UniProt Accession #: P49674
• Molecular Weight: 47,315 Da
• NCBI Official Full Name: casein kinase I isoform epsilon
• NCBI Official Synonym Full Names: casein kinase 1, epsilon
• NCBI Official Symbol: CSNK1E
• NCBI Official Synonym Symbols: HCKIE; CKIepsilon
• NCBI Protein Information: casein kinase I isoform epsilon; CKIe; CKI-epsilon
• UniProt Protein Name: Casein kinase I isoform epsilon
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1E
• UniProt Synonym Gene Names: CKI-epsilon; CKIe
• UniProt Entry Name: KC1E_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CK1E: an ubiquitous protein kinase of the CK1 family. Together with Dvl-1 and Frat-1 activate the Wnt signaling pathway. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. May play a role in cell cycle progression. Two splice variant isoforms have been described. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CK1-D and/or CK1-E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. Interacts with SOCS3. Mutations in hamster and Drosophila orthologs have circadian rhythm phenotypes, and the circadian gene period (per) is a substrate in both human and fly. A coding SNP variant in human, which increases CK1 activity, is negatively associated with circadian disorder. LOF mutations and LOH seen in mammary ductal carcinoma.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CK1; EC 2.7.11.1; CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; ribonucleoprotein complex; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: circadian rhythm; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; organelle organization and biogenesis; endocytosis; DNA repair; regulation of circadian rhythm; signal transduction; protein amino acid phosphorylation; regulation of cell shape; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; circadian regulation of gene expression; G2/M transition of mitotic cell cycle