Mouse anti-Human CSNK1E / CK1 Epsilon Monoclonal Antibody | anti-CSNK1E antibody

Anti-CSNK1E / CK1 Epsilon Antibody (clone 2E1) IHC-plus

Cat. Num. AAA249426

• Gene Names: CSNK1E; HCKIE; CKIepsilon
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Protein A purified
• Synonyms: CSNK1E / CK1 Epsilon; Monoclonal Antibody; Anti-CSNK1E / CK1 Epsilon Antibody (clone 2E1) IHC-plus; Mouse Monoclonal [clone 2E1] (IgG2a; k) to Human CSNK1E / CK1 Epsilon; Human CSNK1E / CK1 Epsilon; Casein kinase 1 epsilon; Casein kinase i epsilon; CK1 epsilon; CKIE; CKIepsilon; CKI-epsilon; HCKIE; Casein kinase 1; epsilon; anti-CSNK1E antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2E1
• Specificity: Human CSNK1E / CK1 Epsilon
• Purity/Purification: Protein A purified
• Form/Format: PBS, pH 7.4.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 416

• Applicable Applications for anti-CSNK1E antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IF (10 ug/ml); IHC-P (10 ug/ml)
• Immunogen: CSNK1E / CK1 Epsilon antibody was raised against cSNK1E (NP_689407, aa2-100 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Description: CSNK1E (NP_689407, aa2-100 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: GST fusion protein
• Target Species: Human
• Preparation and Storage: Store at -20 degree C or lower.; Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry - Paraffin (IHC)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry - Paraffin (IHC)

(Human Colon, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to CSNK1E on HeLa cell. [antibody concentration 10 ug/ml])

Related Product Information for anti-CSNK1E antibody

CSNK1E Antibody, Casein kinase 1 epsilon Antibody, Casein kinase i epsilon Antibody, CK1 epsilon Antibody, CKIE Antibody, CKIepsilon Antibody, CKI-epsilon Antibody, HCKIE Antibody, Casein kinase 1, epsilon Antibody Description: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phospohorylation. Controls PER1 and PER2 nuclear transport and degradation.

NCBI and Uniprot Product Information

• NCBI GI #: 4503093
• NCBI GeneID: 1454
• NCBI Accession #: NP_001885.1
• NCBI GenBank Nucleotide #: NM_001894.4
• UniProt Accession #: P49674
• Molecular Weight: 47,315 Da
• NCBI Official Full Name: casein kinase I isoform epsilon
• NCBI Official Synonym Full Names: casein kinase 1 epsilon
• NCBI Official Symbol: CSNK1E
• NCBI Official Synonym Symbols: HCKIE; CKIepsilon
• NCBI Protein Information: casein kinase I isoform epsilon
• UniProt Protein Name: Casein kinase I isoform epsilon
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1E
• UniProt Synonym Gene Names: CKI-epsilon; CKIe
• UniProt Entry Name: KC1E_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CK1E: an ubiquitous protein kinase of the CK1 family. Together with Dvl-1 and Frat-1 activate the Wnt signaling pathway. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. May play a role in cell cycle progression. Two splice variant isoforms have been described. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CK1-D and/or CK1-E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. Interacts with SOCS3. Mutations in hamster and Drosophila orthologs have circadian rhythm phenotypes, and the circadian gene period (per) is a substrate in both human and fly. A coding SNP variant in human, which increases CK1 activity, is negatively associated with circadian disorder. LOF mutations and LOH seen in mammary ductal carcinoma.

Protein type: Protein kinase, CK1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: ATP binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: circadian regulation of gene expression; circadian rhythm; DNA repair; G2/M transition of mitotic cell cycle; mitotic cell cycle; negative regulation of protein binding; negative regulation of Wnt receptor signaling pathway; organelle organization and biogenesis; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of circadian rhythm; signal transduction; Wnt receptor signaling pathway