Western Blot (WB)
(Western Blot Analysis: Mouse liver lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-RBP-Jk (1:1,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RBP-Jk (55.7kD))
Rabbit anti-Human, Mouse CSL Polyclonal Antibody | anti-CSL antibody
CSL (CBF1, RBP-Jk, J kappa-Recombination Signal-Binding Protein, Suppressor of Hairless, Lag-1) (PE)
Gene Names
RBPJ; SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
CSL; Polyclonal Antibody; CSL (CBF1; RBP-Jk; J kappa-Recombination Signal-Binding Protein; Suppressor of Hairless; Lag-1) (PE); anti-CSL antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the Beta-Trefoil Domain of human and mouse RBP-jk. Species sequence homology: Drosophila, Hydra, Horse, rat, Danio, and chicken.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-CSL antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Synthetic Linear peptide corresponding to the Beta-Trefoil Domain a.a. 194-206 of RBP-jk (KLH).
Conjugate
PE
Positive Control
HepG2 and mouse liver cell lysates.
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot Analysis: Mouse liver lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-RBP-Jk (1:1,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RBP-Jk (55.7kD))
Western Blot (WB)
(Western Blot Analysis: Mouse liver lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-RBP-Jk (1:1,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RBP-Jk (55.7kD))
Testing Data
(HepG2 lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-RBP-Jk. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RBP-Jk (55.7kD))
Testing Data
(HepG2 lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-RBP-Jk. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RBP-Jk (55.7kD))
Product Categories/Family for anti-CSL antibody
References
1. Lendahl, U et al., (1999). Development. 126:3925-3935.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,297 Da
NCBI Official Full Name
recombining binding protein suppressor of hairless isoform 1
NCBI Official Synonym Full Names
recombination signal binding protein for immunoglobulin kappa J region
NCBI Official Symbol
RBPJ
NCBI Official Synonym Symbols
SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1
NCBI Protein Information
recombining binding protein suppressor of hairless; CBF-1; RBP-JK; RBP-J kappa; H-2K binding factor-2; suppressor of hairless homolog; renal carcinoma antigen NY-REN-30; immunoglobulin kappa J region recombination signal binding protein 1
UniProt Protein Name
Recombining binding protein suppressor of hairless
UniProt Gene Name
RBPJ
UniProt Synonym Gene Names
IGKJRB; IGKJRB1; RBPJK; RBPSUH; RBP-J; RBP-JK
UniProt Entry Name
SUH_HUMAN
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Product Notes
The CSL rbpj (Catalog #AAA6470743) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CSL (CBF1, RBP-Jk, J kappa-Recombination Signal-Binding Protein, Suppressor of Hairless, Lag-1) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CSL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CSL rbpj for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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