Rabbit CSK Polyclonal Antibody | anti-CSK antibody

CSK (Phospho-Tyr304) Antibody

Cat. Num. AAA9384367

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: CSK; Polyclonal Antibody; CSK (Phospho-Tyr304) Antibody; anti-CSK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CSK (Phospho-Tyr304) Ab detects endogenous levels of CSK only when phosphorylated at Tyr304.
• Purity/Purification: The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-CSK antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
• Immunogen: A synthesized peptide derived from human CSK around the phosphorylation site of Tyr304.
• Subcellular Location: Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
• Tissue Specificity: Expressed in lung and macrophages.
• Similarity: The architecture of this protein is similar to that of Src-family kinases (SFKs) with one N-terminal SH3 domain, one SH2 domain, and a C-terminal kinase domain. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
• Important: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of CSK (Phospho-Tyr304) using Insulin treated HeLa whole cell lysates.-/+ means absence or presence of N peptide (non-phospho-peptide) and P peptide(phospho peptide).)

Immunohistochemistry (IHC)

(At 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)

Immunohistochemistry (IHC)

(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)

Immunohistochemistry (IHC)

(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)

Immunohistochemistry (IHC)

(At 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)

Immunohistochemistry (IHC)

(At 1/200 staining Mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)

Immunohistochemistry (IHC)

(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)

Immunofluorescence (IF)

(Staining HeLa cells by IF. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary Ab was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG(H+L) Ab(Red), diluted at 1/600, was used as secondary Ab.)

NCBI and Uniprot Product Information

• NCBI GI #: 187475373
• NCBI GeneID: 1445
• NCBI Accession #: NP_001120662.1
• NCBI GenBank Nucleotide #: NM_001127190.1
• UniProt Accession #: P41240; Q2M3N2; Q6FGZ6
• Molecular Weight: 55 KD
• NCBI Official Full Name: tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: CSK, non-receptor tyrosine kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK

Uniprot Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Csk family; EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; plasma membrane

Molecular Function: identical protein binding; kinase activity; non-membrane spanning protein tyrosine kinase activity; protein binding; protein C-terminus binding; protein-tyrosine kinase activity; receptor binding

Biological Process: cell differentiation; cell migration; central nervous system development; epidermal growth factor receptor signaling pathway; innate immune response; negative regulation of Golgi to plasma membrane protein transport; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cytokine production; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway