Rabbit anti-Human, Mouse DVL1 Polyclonal Antibody | anti-DVL1 antibody

DVL1, ID (DVL1, Segment polarity protein dishevelled homolog DVL-1, DSH homolog 1) (FITC)

Cat. Num. AAA6293898

• Gene Names: DVL1; DVL; DVL1L1; DVL1P1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: DVL1; Polyclonal Antibody; ID (DVL1; Segment polarity protein dishevelled homolog DVL-1; DSH homolog 1) (FITC); DSH homolog 1; anti-DVL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DVL1. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DVL1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 442-470 in the central region of human DVL1. Species sequence homology: rat
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB435 cell line lysates (35ug/lane) usingMBS6004027. This demonstrates MBS6004027 detected the DVL1 protein (arrow).)

Testing Data

(All lanes : MBS6004027 at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: human kidney lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size : 75kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS6004027 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6004027 for immunohistochemistry.)

Product Categories/Family for anti-DVL1 antibody

Antibodies; Disease Markers

References

1. Metcalfe, C., et al. J. Cell. Sci. 123 (PT 9), 1588-1599 (2010). 2. Hu, T., et al. J. Biol. Chem. 285(18):13561-13568(2010). 3. Varelas, X., et al. Dev. Cell 18(4):579-591(2010). 4. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010). 5. Guo, J., et al. PLoS ONE 4 (11), E7982 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 32479521
• NCBI GeneID: 1855
• NCBI Accession #: NP_004412.2
• NCBI GenBank Nucleotide #: NM_004421.2
• UniProt Accession #: O14640; P54792
• Molecular Weight: 72,427 Da
• NCBI Official Full Name: segment polarity protein dishevelled homolog DVL-1
• NCBI Official Synonym Full Names: dishevelled segment polarity protein 1
• NCBI Official Symbol: DVL1
• NCBI Official Synonym Symbols: DVL; DVL1L1; DVL1P1
• NCBI Protein Information: segment polarity protein dishevelled homolog DVL-1; DSH homolog 1; dishevelled-1; dishevelled, dsh homolog 1; dishevelled 1 (homologous to Drosophila dsh)
• UniProt Protein Name: Putative segment polarity protein dishevelled homolog DVL1P1
• Protein Family: Segment polarity protein dishevelled
• UniProt Gene Name: DVL1P1
• UniProt Synonym Gene Names: DVL; DVL1; DVL1L1; Dishevelled-1-like
• UniProt Entry Name: DVLP1_HUMAN

NCBI Description

DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development. [provided by RefSeq, Jul 2008]

Uniprot Description

DVL1: Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Belongs to the DSH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: microtubule; growth cone; cell soma; clathrin-coated vesicle; axon; cytoplasm; cytoplasmic membrane-bound vesicle; dendrite; synapse; cytoplasmic vesicle; cytosol; lateral plasma membrane

Molecular Function: identical protein binding; protein binding; enzyme binding; frizzled binding; Rac GTPase binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; neural tube development; axon guidance; Wnt receptor signaling pathway; prepulse inhibition; convergent extension involved in neural plate elongation; axon extension; Wnt receptor signaling pathway, planar cell polarity pathway; dendrite morphogenesis; neurotransmitter secretion; regulation of neurotransmitter levels; heart development; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; social behavior; receptor clustering; cytoplasmic microtubule organization and biogenesis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; collateral sprouting; synapse organization and biogenesis; negative regulation of protein kinase activity; negative regulation of protein binding; positive regulation of Wnt receptor signaling pathway; neuromuscular junction development

Disease: Robinow Syndrome, Autosomal Dominant; Robinow Syndrome, Autosomal Dominant 2