Goat CSK Polyclonal Antibody | anti-CSK antibody

GOAT ANTI HUMAN CSK (C-TERMINAL)

Cat. Num. AAA221702

• Reactivity: Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: CSK; Polyclonal Antibody; GOAT ANTI HUMAN CSK (C-TERMINAL); anti-CSK antibody

• Host: Goat
• Reactivity: Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises an epitope within the C-terminal (CT) region of CSK (c-src tyrosine kinase), a cytoplasmic tyrosine kinase ubiquitously expressed as two mRNA species, which down-regulates the activity of the c-src oncoprotein.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 450

• Applicable Applications for anti-CSK antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 50kDa in Jurkat cell lysates. ; ELISA: Maximum Dilution: >1/32,000; Immunohistology - Paraffin: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.3ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.; TRIS/EDTA buffer pH 9.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5ug/ml; Maximum Dilution: 2.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide; 0.5% Bovine Serum Albumin
• Immunogen: Peptide sequence EQLEHIKTHELHL corresponding to the C-terminal region of CSK (NP_004374.1).; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to human CSK (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CSK antibody

MBS221702 specifically recognises an epitope within the C-terminal (CT) region of CSK (c-src tyrosine kinase), a cytoplasmic tyrosine kinase ubiquitously expressed as two mRNA species, which down-regulates the activity of the c-src oncoprotein.

NCBI and Uniprot Product Information

• NCBI GI #: 187475373
• NCBI GeneID: 1445
• NCBI Accession #: NP_001120662.1
• NCBI GenBank Nucleotide #: NM_001127190.1
• UniProt Accession #: P41240; Q2M3N2; Q6FGZ6
• Molecular Weight: 50,704 Da
• NCBI Official Full Name: tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: c-src tyrosine kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK; C-Src kinase; protein-tyrosine kinase CYL
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK
• UniProt Entry Name: CSK_HUMAN

Uniprot Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; TK group; Csk family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of phagocytosis; platelet activation; negative regulation of kinase activity; cell migration; central nervous system development; negative regulation of Golgi to plasma membrane protein transport; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of cytokine production; regulation of cell proliferation; positive regulation of MAP kinase activity; negative regulation of cell proliferation; T cell costimulation; negative regulation of interleukin-6 production; innate immune response; oligodendrocyte differentiation; morphogenesis of an epithelium; brain development; cell differentiation; blood coagulation; negative regulation of bone resorption; transmembrane receptor protein tyrosine kinase signaling pathway