Rabbit anti-Human NLRP6 Polyclonal Antibody | anti-NLRP6 antibody

NLRP6, NT (NLRP6, NALP6, PYPAF5, NACHT, LRR and PYD domains-containing protein 6, Angiotensin II/vasopressin receptor, PYRIN-containing APAF1-like protein 5) (FITC)

Cat. Num. AAA6325799

• Gene Names: NLRP6; PAN3; NALP6; PYPAF5; CLR11.4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NLRP6; Polyclonal Antibody; NT (NLRP6; NALP6; PYPAF5; NACHT; LRR and PYD domains-containing protein 6; Angiotensin II/vasopressin receptor; PYRIN-containing APAF1-like protein 5) (FITC); PYRIN-containing APAF1-like protein 5; anti-NLRP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NLRP6 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NLRP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-197 amino acids from the N-terminal region of human NLRP6.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NLRP6Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NLRP6 antibody detected the NLRP6 protein (arrow).)

Related Product Information for anti-NLRP6 antibody

NALPs are cytoplasmic proteins that form a subfamily within the larger CATERPILLER protein family. Most short NALPs, such as NALP6, have an N-terminal pyrin (MEFV; MIM 608107) domain (PYD), followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. The long NALP, NALP1 (MIM 606636), also has a C-terminal extension containing a function to find domain (FIIND) and a caspase recruitment domain (CARD). NALPs are implicated in the activation of proinflammatory caspases (e.g., CASP1; MIM 147678) via their involvement in multiprotein complexes called inflammasomes (Tschopp et al., 2003 [PubMed 12563287]).

Product Categories/Family for anti-NLRP6 antibody

Antibodies; Inflammasomes

References

Cummings, J.R., et al. Tissue Antigens 76(1):48-56(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Albrecht, M., et al. FEBS Lett. 538 (1-3), 173-177 (2003): Tschopp, J., et al. Nat. Rev. Mol. Cell Biol. 4(2):95-104(2003). Grenier, J.M., et al. FEBS Lett. 530 (1-3), 73-78 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 21264320
• NCBI GeneID: 171389
• NCBI Accession #: NP_612202.1
• NCBI GenBank Nucleotide #: NM_138329.1
• UniProt Accession #: P59044; A8K9F3
• Molecular Weight: 98,768 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 6
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 6
• NCBI Official Symbol: NLRP6
• NCBI Official Synonym Symbols: PAN3; NALP6; PYPAF5; CLR11.4
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 6; angiotensin II/vasopressin receptor; PYRIN-containing APAF1-like protein 5; NACHT, LRR and PYD containing protein 6; NACHT, leucine rich repeat and PYD containing 6; nucleotide-binding oligomerization domai
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 6
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP6
• UniProt Synonym Gene Names: NALP6; PYPAF5
• UniProt Entry Name: NALP6_HUMAN

Uniprot Description

NLRP6: May contribute to arginine-vasopressin (AVP)-mediated regulation of renal salt-water balance, glucose and lipid metabolism, apoptosis, and/or cell cycle. Binds arginine- vasopressin but not angiotensin II and acts as an arginine- vasopressin V2 receptor. Plays a role in modulating inflammatory responses in the colon to allow recovery from intestinal epithelial damage and limit tumorigenic potential. Protects against the development of colitis by controlling the composition of the gut microbiota by preventing colonization of the colon by harmful, inflammation-inducing bacteria such as Prevotella species. May mediate activation of CASP1 via ASC, promote activation of NF-kappa-B and stimulate cAMP accumulation. Belongs to the NLRP family.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: nuclear membrane; cytoplasm; plasma membrane

Molecular Function: ATP binding; vasopressin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; wound healing; response to bacterium; negative regulation of MAPKKK cascade; regulation of inflammatory response; negative regulation of immune response; negative regulation of toll-like receptor signaling pathway; regulation of autophagy; negative regulation of I-kappaB kinase/NF-kappaB cascade