Canine Contactin 1 ELISA Kit | CNTN1 elisa kit

Canine Contactin 1 ELISA Kit

Cat. Num. AAA738433

• Gene Names: CNTN1; F3; GP135; MYPCN
• Reactivity: Canine
• Synonyms: Contactin 1; Canine Contactin 1 ELISA Kit; CNTN1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This CNTN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CNTN1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNTN1 elisa kit

Principle of the Assay: CNTN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNTN1 antibody and an CNTN1-HRP conjugate. The assay sample and buffer are incubated together with CNTN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNTN1 concentration since CNTN1 from samples and CNTN1-HRP conjugate compete for the anti-CNTN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNTN1 from the sample, fewer sites are left to bind CNTN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNTN1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CNTN1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 28373119
• NCBI GeneID: 1272
• NCBI Accession #: NP_778203.1
• NCBI GenBank Nucleotide #: NM_175038.2
• Molecular Weight: 70,604 Da
• NCBI Official Full Name: contactin-1 isoform 2
• NCBI Official Synonym Full Names: contactin 1
• NCBI Official Symbol: CNTN1
• NCBI Official Synonym Symbols: F3; GP135; MYPCN
• NCBI Protein Information: contactin-1; glycoprotein gP135; neural cell surface protein F3
• UniProt Protein Name: Contactin-1
• Protein Family: Contactin
• UniProt Gene Name: CNTN1
• UniProt Entry Name: CNTN1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CNTN1: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. Defects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM). CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia. Belongs to the immunoglobulin superfamily. Contactin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 12q11-q12

Cellular Component: membrane; plasma membrane

Molecular Function: glycoprotein binding; carbohydrate binding

Biological Process: axon guidance; Notch signaling pathway; cerebellum development; cell adhesion

Disease: Myopathy, Congenital, Compton-north