Rabbit Cry1 Polyclonal Antibody | anti-CRY1 antibody

Cry1 Antibody (C-term)

Cat. Num. AAA9208683

• Gene Names: CRY1; PHLL1
• Reactivity: Human (Predicted Reactivity: Monkey)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Cry1; Polyclonal Antibody; Cry1 Antibody (C-term); Cryptochrome-1; CRY1; PHLL1; anti-CRY1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Monkey)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Cry1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 556-586 amino acids from the C-terminal region of human Cry1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 556-586
• Sequence Length: 586

• Applicable Applications for anti-CRY1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-Cry1 N-term Pab is used in Western blot to detect Cry1 in placenta tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-CRY1 antibody

Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central ?gears?driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

Product Categories/Family for anti-CRY1 antibody

Cardiovascular; Neuroscience

References

Griffin, E.A. Jr., et al., Science 286(5440):768-771 (1999).
van der Horst, G.T., et al., Nature 398(6728):627-630 (1999).
Kobayashi, K., et al., Nucleic Acids Res. 26(22):5086-5092 (1998).
Hsu, D.S., et al., Biochemistry 35(44):13871-13877 (1996).
van der Spek, P.J., et al., Genomics 37(2):177-182 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4758072
• NCBI GeneID: 1407
• NCBI Accession #: NP_004066.1
• NCBI GenBank Nucleotide #: NM_004075.4
• UniProt Accession #: Q16526
• Molecular Weight: 66395
• NCBI Official Full Name: cryptochrome-1
• NCBI Official Synonym Full Names: cryptochrome circadian clock 1
• NCBI Official Symbol: CRY1
• NCBI Official Synonym Symbols: PHLL1
• NCBI Protein Information: cryptochrome-1
• UniProt Protein Name: Cryptochrome-1
• Protein Family: Cryptochrome
• UniProt Gene Name: CRY1
• UniProt Synonym Gene Names: PHLL1
• UniProt Entry Name: CRY1_HUMAN

NCBI Description

This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Jan 2014]

Uniprot Description

CRY1: Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 C-terminal domains. Interaction with PER2 inhibits its ubiquitination and vice versa. Binds MAPK. Interacts with FBXL21. Interacts with FBXL3. Expression is regulated by light and circadian rhythms. Peak expression in the suprachiasma nucleus (SCN) and eye at the day/night transition (CT12). Levels decrease with ARNTL-CLOCK inhibition as part of the autoregulatory feedback loop. Belongs to the DNA photolyase class-1 family.

Protein type: Lyase; Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: mitochondrion; nucleus

Molecular Function: ubiquitin binding; histone deacetylase binding; DNA (6-4) photolyase activity; nucleotide binding; protein kinase binding; transcription factor binding; phosphatase binding; protein binding; DNA binding; double-stranded DNA binding; deoxyribodipyrimidine photo-lyase activity; blue light photoreceptor activity; nuclear hormone receptor binding

Biological Process: circadian rhythm; response to glucagon stimulus; transcription, DNA-dependent; DNA damage induced protein phosphorylation; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; DNA repair; glucose homeostasis; protein-chromophore linkage; response to insulin stimulus; entrainment of circadian clock by photoperiod; gluconeogenesis; blue light signaling pathway; sequestering of lipid; negative regulation of circadian rhythm; negative regulation of protein ubiquitination; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent