Rabbit anti-Human CRY1 Polyclonal Antibody | anti-CRY1 antibody

CRY1, CT (Cryptochrome-1, PHLL1) (APC)

Cat. Num. AAA6470701

• Gene Names: CRY1; DSPD; PHLL1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CRY1; Polyclonal Antibody; CT (Cryptochrome-1; PHLL1) (APC); anti-CRY1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Cry1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CRY1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide C-terminal region of human Cry1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-Cry1 N-term Pab is used in Western blot to detect Cry1 in placenta tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CRY1 antibody

Antibodies; Receptors

References

1. Griffin, E.A. Jr., et al., Science 286(5440):768-771 (1999). 2. van der Horst, G.T., et al., Nature 398(6728):627-630 (1999). 3. Kobayashi, K., et al., Nucleic Acids Res. 26(22):5086-5092 (1998). 4. Hsu, D.S., et al., Biochemistry 35(44):13871-13877 (1996). 5. van der Spek, P.J., et al., Genomics 37(2):177-182 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4758072
• NCBI GeneID: 1407
• NCBI Accession #: NP_004066
• NCBI GenBank Nucleotide #: NM_004075
• UniProt Accession #: Q16526
• Molecular Weight: 64kDa
• NCBI Official Full Name: cryptochrome-1
• NCBI Official Synonym Full Names: cryptochrome circadian regulator 1
• NCBI Official Symbol: CRY1
• NCBI Official Synonym Symbols: DSPD; PHLL1
• NCBI Protein Information: cryptochrome-1
• UniProt Protein Name: Cryptochrome-1
• Protein Family: Cryptochrome
• UniProt Gene Name: CRY1
• UniProt Synonym Gene Names: PHLL1
• UniProt Entry Name: CRY1_HUMAN

NCBI Description

This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Jan 2014]

Uniprot Description

CRY1: Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 C-terminal domains. Interaction with PER2 inhibits its ubiquitination and vice versa. Binds MAPK. Interacts with FBXL21. Interacts with FBXL3. Expression is regulated by light and circadian rhythms. Peak expression in the suprachiasma nucleus (SCN) and eye at the day/night transition (CT12). Levels decrease with ARNTL-CLOCK inhibition as part of the autoregulatory feedback loop. Belongs to the DNA photolyase class-1 family.

Protein type: Lyase; DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: mitochondrion; nucleus

Molecular Function: ubiquitin binding; protein binding; DNA binding; DNA (6-4) photolyase activity; histone deacetylase binding; double-stranded DNA binding; nucleotide binding; deoxyribodipyrimidine photo-lyase activity; transcription factor binding; blue light photoreceptor activity; protein kinase binding; phosphatase binding; nuclear hormone receptor binding

Biological Process: circadian rhythm; response to glucagon stimulus; transcription, DNA-dependent; DNA damage induced protein phosphorylation; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of transcription from RNA polymerase II promoter; DNA repair; regulation of circadian rhythm; glucose homeostasis; protein-chromophore linkage; response to insulin stimulus; entrainment of circadian clock by photoperiod; gluconeogenesis; blue light signaling pathway; sequestering of lipid; negative regulation of circadian rhythm; negative regulation of protein ubiquitination; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent