Rabbit anti-Human, Mouse CRTC1 Polyclonal Antibody | anti-CRTC1 antibody

CRTC1 Antibody

Cat. Num. AAA9603929

• Gene Names: CRTC1; MAML2; MECT1; Mam-2; TORC1; TORC-1; WAMTP1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CRTC1; Polyclonal Antibody; CRTC1 Antibody; KIAA0616; CREB regulated transcription coactivator 1; CREB-regulated transcription coactivator 1; CRTC1_HUMAN; FLJ14027; MECT 1; Mucoepidermoid carcinoma translocated 1; Mucoepidermoid carcinoma translocated protein 1; TORC-1; TORC1; Transducer of CREB protein 1; Transducer of regulated cAMP response element binding protein 1; Transducer of regulated cAMP response element-binding protein (CREB) 1; Transducer of regulated cAMP response element-binding protein 1; WAMTP1; anti-CRTC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CRTC1 antibody detects endogenous levels of total CRTC1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 650

• Applicable Applications for anti-CRTC1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide
• Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity).
• Tissue Specificity: Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
• Conjugation: Unconjugated

Western Blot (WB)

(Western blot analysis of CRTC1 expression in Fetal brain lysate; C6 cell lysate, The lane on the left is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9603929 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-CRTC1 antibody

Function: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.
Subunit Structure: Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 (PubMed:14536081). 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction (PubMed:14536081). Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1 (PubMed:14536081). Binds HTLV1 Tax. Interacts with 14-3-3 proteins (By similarity).
Post-translational Modifications: Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1 (By similarity).
Similarity: Belongs to the TORC family.

NCBI and Uniprot Product Information

• NCBI GI #: 148596988
• NCBI GeneID: 23373
• NCBI Accession #: NP_001091952.1
• NCBI GenBank Nucleotide #: NM_001098482.1
• UniProt Accession #: Q6UUV9; O75114; Q6Y3A3; Q7LDZ2; Q8IUL3; Q8IZ34; Q8IZL1; Q8N6W3; Q96AI8; Q9H801; A6NMG5
• Molecular Weight: Observed: 78 kDa; Predicted: 68 kDa
• NCBI Official Full Name: CREB-regulated transcription coactivator 1 isoform 3
• NCBI Official Synonym Full Names: CREB regulated transcription coactivator 1
• NCBI Official Symbol: CRTC1
• NCBI Official Synonym Symbols: MAML2; MECT1; Mam-2; TORC1; TORC-1; WAMTP1
• NCBI Protein Information: CREB-regulated transcription coactivator 1
• UniProt Protein Name: CREB-regulated transcription coactivator 1
• Protein Family: CREB-regulated transcription coactivator
• UniProt Gene Name: CRTC1
• UniProt Synonym Gene Names: TORC-1; Transducer of CREB protein 1

Uniprot Description

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.