Rabbit CREB Polyclonal Antibody | anti-CREB1 antibody

CREB, phosphorylated (Ser133) (cAMP Response Element Binding Protein)

Cat. Num. AAA630215

• Gene Names: CREB1; CREB; MGC9284
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CREB; Polyclonal Antibody; phosphorylated (Ser133) (cAMP Response Element Binding Protein); Anti -CREB; anti-CREB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes CREB, phosphorylated at Ser133. Species Crossreactivity: Human, rat and mouse.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM aodium HEPES, pH 7.5, 150mM sodium chloride, 100ug BSA and 50% glycerol.

• Applicable Applications for anti-CREB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation.; Dilution: Immunohistochemistry:
• Immunogen: Phosphopeptide corresponding to residues surrounding Ser133 of human CREB
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Confocal images of double immunostaining with Phospho-CREB (Ser133) Antibody (green) and calbindin antibody (red) in the hippocampus using the sections from control and 24 hours of reperfusion following 15 minute transient cerebral ischemia in rats. Yellow represents overlay of red and green.)

Product Categories/Family for anti-CREB1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 33694240
• NCBI GeneID: 1385
• UniProt Accession #: Q6V963; P16220; Q16366; Q16367; Q4ZG78; Q53RU9; Q53X93; Q5U0J5
• Molecular Weight: 25,445 Da
• NCBI Official Full Name: CREB
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: CREB1
• NCBI Official Synonym Symbols: CREB; MGC9284
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1; CREB-1; OTTHUMP00000163864; OTTHUMP00000163865; OTTHUMP00000206660; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; active transcription factor CREB; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1
• UniProt Protein Name: CREB
• Protein Family: CREB-binding protein
• UniProt Entry Name: Q6V963_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; transcription cofactor activity; transcription factor activity

Biological Process: circadian rhythm; lactation; transcription from RNA polymerase II promoter; axon guidance; response to glucagon stimulus; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; positive regulation of multicellular organism growth; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; response to organic substance; synaptic transmission; toll-like receptor 4 signaling pathway; response to drug; epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; Notch signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; protein stabilization; secretory granule organization and biogenesis; organelle organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; memory; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; pituitary gland development; phospholipase C activation; toll-like receptor signaling pathway; innate immune response; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; regulation of cell size

Disease: Histiocytoma, Angiomatoid Fibrous