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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human Kell Protein Polyclonal Antibody | anti-KEL antibody

Kell Protein (KEL) Polyclonal Antibody

Gene Names
KEL; ECE3; CD238
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Kell Protein; Polyclonal Antibody; Kell Protein (KEL) Polyclonal Antibody; CD238; ECE3; Kell Blood Group; Metalloendopeptidase; Metallo-Endopeptidase; Kell blood group glycoprotein; anti-KEL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against KEL. It has been selected for its ability to recognize KEL in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
732
Applicable Applications for anti-KEL antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
KEL (Arg128~Ser332)
Quality Control
Content: The quality control contains recombinant KEL (Arg128~Ser332) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2058215
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,824 Da
NCBI Official Full Name
kell blood group glycoprotein
NCBI Official Synonym Full Names
Kell blood group, metallo-endopeptidase
NCBI Official Symbol
KEL
NCBI Official Synonym Symbols
ECE3; CD238
NCBI Protein Information
kell blood group glycoprotein
UniProt Protein Name
Kell blood group glycoprotein
UniProt Gene Name
KEL

NCBI Description

This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases. [provided by RefSeq, Jul 2008]

Uniprot Description

KEL: Zinc endopeptidase with endothelin-3-converting enzyme activity. Cleaves EDN1, EDN2 and EDN3, with a marked preference for EDN3. Belongs to the peptidase M13 family.

Protein type: EC 3.4.24.-; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: integral component of membrane; plasma membrane

Molecular Function: metal ion binding; metalloendopeptidase activity; protein binding

Biological Process: cellular calcium ion homeostasis; myelination; proteolysis; regulation of axon diameter; regulation of cell size; skeletal muscle fiber development; vasoconstriction

Disease: Blood Group--kell System

Research Articles on KEL

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Product Notes

The KEL kel (Catalog #AAA2032121) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kell Protein (KEL) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kell Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the KEL kel for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kell Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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