Rabbit CREB Polyclonal Antibody | anti-CREB antibody

CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein) (AP)

Cat. Num. AAA6470590

• Gene Names: Creb1; Creb
• Reactivity: Rat, Hamster, Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Purified by Dual Antigen affinity chromatography.
• Synonyms: CREB; Polyclonal Antibody; phosphorylated; Ser133 (cAMP Response Element Binding Protein) (AP); anti-CREB antibody

• Host: Rabbit
• Reactivity: Rat, Hamster, Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat CREB when phosphorylated on Ser133, Mr ~43kD. May recognize ATF-1 and CREM due to signifigant homology to phosphorylated immunizing peptide. Species Crossreactivity: mouse, human and hamster
• Purity/Purification: Purified by Dual Antigen affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CREB antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
• Application Notes: IHC-P: 1:1000 dilution detects phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.; WB: 1:1000 Detected phosphorylated CREB in RIPA lysates from mouse 3T3 cells treated with 50mM forskolin. PC-12 and forskolin treated PC-12 cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-CREB (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.; IP: 10ul immunoprecipitates phosphorylated CREB from 500ug of rat PC-12 cells treated with 50uM forskolin; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acids including and emcompassing phosphorylated Ser133 of human CREB conjugated to KLH (P16220).
• Conjugate: AP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WB: untreated (lane 1) and forskolin treated (lane 2) 3T3 cell lysates were probed with MBS611737 (1:1000). )

Product Categories/Family for anti-CREB antibody

Antibodies; Transcription Factors

References

1. Ginty, et al., (1993) Science 260: 238-241. 2. Lei, Z., and Rao, C.V, (1997). J. Biol. Chem. 272: 14365-14371, 1997. 3. Le Tulzo, Y., et al., (1997). J. Clin. Invest. 99: 1516-1524, 1997. 4. Wu, G. Y., et al. (2001). USA., 98: 2808-2813. 5. Samato, H., et al. (2003). 278: 28659-28667.

NCBI and Uniprot Product Information

• NCBI GI #: 13591924
• NCBI GeneID: 81646
• NCBI Accession #: NP_112279.1
• NCBI GenBank Nucleotide #: NM_031017.1
• UniProt Accession #: P15337
• Molecular Weight: 35,081 Da
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 1 isoform B
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: Creb1
• NCBI Official Synonym Symbols: Creb
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1; CREB-1; Y protein; cAMP response element binding protein 1; cAMP-responsive element-binding protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 1
• Protein Family: CREB-binding protein
• UniProt Gene Name: Creb1
• UniProt Synonym Gene Names: Creb-1; CREB-1; cAMP-responsive element-binding protein 1
• UniProt Entry Name: CREB1_RAT

NCBI Description

binds the cAMP response element in many gene promoters and regulates transcription [RGD, Feb 2006]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; transcription factor complex; mitochondrion; nuclear chromatin; chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; double-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; lactation; response to glucagon stimulus; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; positive regulation of multicellular organism growth; protein amino acid phosphorylation; regulation of apoptosis; response to organic substance; regulation of transcription, DNA-dependent; mammary gland development; transforming growth factor beta receptor signaling pathway; positive regulation of transforming growth factor-beta3 production; positive regulation of transcriptional preinitiation complex assembly; cell differentiation; response to drug; protein stabilization; secretory granule organization and biogenesis; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; regulation of circadian rhythm; memory; axonogenesis; pituitary gland development; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell size