Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolactin receptor (PRLR) Standard Curve.)
Mouse Prolactin Receptor (PRLR) ELISA Kit | PRLR elisa kit
Mouse Prolactin Receptor (PRLR) ELISA Kit
Gene Names
Prlr; Pr-1; Pr-3; AI987712; Prlr-rs1
Reactivity
Mouse
Synonyms
Prolactin Receptor (PRLR); Mouse Prolactin Receptor (PRLR) ELISA Kit; PRLR; hPRLrI; delta 4-SF1b truncated prolactin receptor; delta 4-delta 7/11 truncated prolactin receptor; hPRL receptor; prolactin receptor delta 7/11; secreted prolactin binding protein; PRLR elisa kit
Reactivity
Mouse
Specificity
Mouse Prolactin receptor (PRLR) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRLR. No significant cross-reactivity or interference between Mouse PRLR and analogues was observed.
Sequence Length
303
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolactin receptor (PRLR) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolactin receptor (PRLR) Standard Curve.)
Related Product Information for PRLR elisa kit
Description: This Mouse Prolactin receptor (PRLR) ELISA Kit employs a two-site sandwich ELISA to quantitate PRLR in samples. An antibody specific for PRLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRLR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRLR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The prolactin receptor—encoded by a gene on Chromosome 5p13-14—interacts with the prolactin molecule as a transmembrane receptor.The 5-prime-untranslated region of the PRLR gene contains 2 alternative first exons: E13, the human counterpart of the rat and mouse E13, and a novel human type of alternative first exon termed E1N. The 5-prime-untranslated region also contains a common noncoding exon 2 and part of exon 3, which contains the translation initiation codon. The E13 and E1N exons are within 800 basepairs of each other. These 2 exons are expressed in human breast tissue, breast cancer cells, gonads, and liver. Overall, the transcript containing E13 is prevalent in most tissues. The PRLR gene product is encoded by exons 3-10, of which exon 10 encodes most of the intracellular domain.
Background: The prolactin receptor—encoded by a gene on Chromosome 5p13-14—interacts with the prolactin molecule as a transmembrane receptor.The 5-prime-untranslated region of the PRLR gene contains 2 alternative first exons: E13, the human counterpart of the rat and mouse E13, and a novel human type of alternative first exon termed E1N. The 5-prime-untranslated region also contains a common noncoding exon 2 and part of exon 3, which contains the translation initiation codon. The E13 and E1N exons are within 800 basepairs of each other. These 2 exons are expressed in human breast tissue, breast cancer cells, gonads, and liver. Overall, the transcript containing E13 is prevalent in most tissues. The PRLR gene product is encoded by exons 3-10, of which exon 10 encodes most of the intracellular domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prolactin receptor isoform 3
NCBI Official Synonym Full Names
prolactin receptor
NCBI Official Symbol
Prlr
NCBI Official Synonym Symbols
Pr-1; Pr-3; AI987712; Prlr-rs1
NCBI Protein Information
prolactin receptor
UniProt Protein Name
Prolactin receptor
Protein Family
UniProt Gene Name
Prlr
UniProt Synonym Gene Names
PRL-R
UniProt Entry Name
PRLR_MOUSE
Uniprot Description
PRLR: receptor for the anterior pituitary hormone prolactin and placental lactogen I and II. Interacts with Cyclophilin A, differentially regulating various signaling pathways from the PrlR. Prolactin signaling is attenuated by threonine and tyrosine phosphorylation. Two alternative splice isoforms have been identified.
Protein type: Receptor, cytokine; Membrane protein, integral
Cellular Component: cell surface; membrane; integral to membrane; intracellular
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; peptide hormone binding; metal ion binding; protein kinase binding; prolactin receptor activity
Biological Process: regulation of cell adhesion; lactation; transmembrane receptor protein tyrosine kinase activation (dimerization); regulation of epithelial cell differentiation; JAK-STAT cascade
Research Articles on PRLR
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Product Notes
The Mouse PRLR prlr (Catalog #AAA9717270) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717270 ELISA Kit recognizes Mouse PRLR. It is sometimes possible for the material contained within the vial of "Prolactin Receptor (PRLR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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