Rabbit anti-Human Creatine Transporter Polyclonal Antibody | anti-SLC6A8 antibody

Creatine Transporter (CT1, CRT)

Cat. Num. AAA616565

• Gene Names: SLC6A8; CRT; CT1; CRTR; MGC87396
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Creatine Transporter; Polyclonal Antibody; Creatine Transporter (CT1; CRT); Anti -Creatine Transporter (CT1; anti-SLC6A8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Human Creatine Transporter. No significant sequence homology with CRT2/CT2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, pH7.2, 0.1% BSA. Reconsitute with 100ul sterile PBS.
• Concentration: ~1mg/ml (after reconstitution) (varies by lot)

• Applicable Applications for anti-SLC6A8 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml (ECL).; ELISA: ~1:100,000 using 50-100ng control peptide/well.; Optimal dilutions to be determined by the researcher.; Control Peptide: Please inquire
• Immunogen: Synthetic peptide 20aa near the N-terminal, cytoplasmic domain of human creatine transporter (KLH).
• Species sequence homology: Rat and Rabbit: 100%; Bovine: 90%
• Preparation and Storage: Lyophilized and reconstituted products are stable for 12 months after receipt at -220 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SLC6A8 antibody

Creatine and creatine phosphate are essential for the maintenance of ATP levels in tissues with high and fluctuating energy demands such as skeletal muscle and brain. Creatine kinase catalyzes the reversible transfer of a phosphate from creatine phosphate to ADP regenerating ATP. In mammals, creatine is either synthesized by sequential reactions occurring in the kidney and liver or obtained from the diet. Muscle cells do not synthesize creatine. They take it up via a special sodium-dependent transporter, the creatine transporter (CRT). Inhibition of creatine transport in experimental animals causes muscle weakness. A specific uptake system for creatine has been demonstrated in skeletal muscle, human monocytes, macrophages and astroglial-rich cultures. CRT exhibits significant homology to the Na+ and Cl- -dependent GABA/norepinephrine (GAT-1/norepinephrine transporter) gene family of neurotransmitter transporters. All members of this family are predicted to contain 12-membrane-spanning domains, to contain a large extracellular loop containing sites for N-linked glycosylation between the third and fourth transmembrane domains, and to have the amino and carboxyl termini facing the cytoplasmic side of the membrane. At least two CRT have been cloned, expressed and characterized. CRT1 or CT1 (mouse, human 635aa, chromosome X28) is expressed in kidney, muscle, brain and other tissues (1). CT2 (human 426aa, chromosome 16p11.2) is testis specific (2).

Product Categories/Family for anti-SLC6A8 antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 765234
• NCBI GeneID: 6535
• UniProt Accession #: P48029; Q66I36; Q13032; Q59EV7
• Molecular Weight: 70,523 Da
• NCBI Official Full Name: creatine transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter, creatine), member 8
• NCBI Official Symbol: SLC6A8
• NCBI Official Synonym Symbols: CRT; CT1; CRTR; MGC87396
• NCBI Protein Information: sodium- and chloride-dependent creatine transporter 1; OTTHUMP00000025945; OTTHUMP00000025946; creatine transporter SLC6A8; solute carrier family 6 member 8
• UniProt Protein Name: Sodium- and chloride-dependent creatine transporter 1
• Protein Family: Creatine transporter
• UniProt Gene Name: SLC6A8
• UniProt Entry Name: SC6A8_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein whose function is to transport creatine into and out of cells. Defects in this gene can result in X-linked creatine deficiency syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

SLC6A8: Required for the uptake of creatine in muscles and brain. Defects in SLC6A8 are the cause of X-linked creatine deficiency syndrome (XL-CDS). XL-CDS causes developmental delay, hypotonia, mental retardation, seizures, short stature and midface hypoplasia. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A8 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, SLC family

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: creatine transporter activity; neurotransmitter:sodium symporter activity; creatine:sodium symporter activity

Biological Process: muscle contraction; neurotransmitter transport; transport; sodium ion transport; creatine metabolic process; creatine transport

Disease: Cerebral Creatine Deficiency Syndrome 1