Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using SPDYA antibody.)
Rabbit SPDYA Polyclonal Antibody | anti-SPDYA antibody
SPDYA Polyclonal Antibody
Gene Names
SPDYA; SPY1; SPDY1; RINGO3; RINGOA
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SPDYA; Polyclonal Antibody; SPDYA Polyclonal Antibody; hSpy/Ringo A; MGC110856; MGC57218; OTTHUMP00000082662; OTTHUMP00000082663; OTTHUMP00000082664; Rapid inducer of G2/M progression in oocytes A; RINGO A; RINGO3; SPDY1; Speedy A; Speedy homolog 1 (Drosophila); Speedy homolog A (Xenopus laevis); Speedy homolog A; Speedy-1; SPY1; anti-SPDYA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SPDYA polyclonal antibody detects endogenous levels of SPDYA protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
313
Applicable Applications for anti-SPDYA antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant full length Human SPDYA.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using SPDYA antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using SPDYA antibody.)
Related Product Information for anti-SPDYA antibody
Speedy A, also known as SPDYA, SPDY1, Ringo3 or SPY1, is a 313 amino acid protein that localizes to the nucleus and belongs to the speedy/ringo family. Expressed at high levels in testis and at lower levels in brain, kidney, heart, bone marrow, colon, lung, liver spleen and placenta, Speedy A functions to regulate the G1/S phase transition of the cell cycle, specifically by binding to and activating Cdc2 p34, Cdk2 and p27. Additionally, Speedy A mediates cell survival during DNA damage, suggesting that Speedy A plays a role in proper cell cycle progression throughout the life of the cell. Multiple isoforms of Speedy A exist due to alternative splicing events. The gene encoding Speedy A maps to human chromosome 2, which encodes over 1, 400 genes and comprises nearly 8% of the human genome.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 48kDa
NCBI Official Full Name
Speedy protein A
NCBI Official Synonym Full Names
speedy/RINGO cell cycle regulator family member A
NCBI Official Symbol
SPDYA
NCBI Official Synonym Symbols
SPY1; SPDY1; RINGO3; RINGOA
NCBI Protein Information
speedy protein A
UniProt Protein Name
Speedy protein A
Protein Family
UniProt Gene Name
SPDYA
UniProt Synonym Gene Names
RINGO A; hSpy/Ringo A; Spy1
UniProt Entry Name
SPDYA_HUMAN
Uniprot Description
SPDYA: Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2. Belongs to the Speedy/Ringo family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 2p23.2
Cellular Component: nucleus
Molecular Function: protein kinase binding
Biological Process: positive regulation of cyclin-dependent protein kinase activity; multicellular organismal development; positive regulation of cell proliferation; male meiosis; response to DNA damage stimulus; G1/S transition of mitotic cell cycle
Research Articles on SPDYA
Similar Products
Product Notes
The SPDYA spdya (Catalog #AAA3008215) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPDYA Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SPDYA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SPDYA spdya for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPDYA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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