Immunohistochemistry (IHC)
(MBS9600530 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human CRBP III Polyclonal Antibody | anti-CRBPIII antibody
CRBP III Antibody
Gene Names
RBP5; CRBP3; CRBPIII; HRBPiso; CRBP-III
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CRBP III; Polyclonal Antibody; CRBP III Antibody; Cellular retinol-binding protein III; CRBP-III; CRBP3; HRBPiso; OTTHUMP00000238163; Putative cellular retinol binding protein CRBP III; RBP5; RET5_HUMAN; Retinol binding protein; Retinol binding protein 5; cellular; Retinol-binding protein 5; anti-CRBPIII antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CRBP III antibody detects endogenous levels of CRBP III
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
135
Applicable Applications for anti-CRBPIII antibody
Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human CRBP III
Subcellular Location
Cytoplasm.
Tissue Specificity
Higher expression in adult kidney and liver and to a lesser extent in adult and fetal spleen, adult lymph nodes and appendix, and fetal liver and kidney. Strongly decreased in hepatocellular carcinoma tissues (at protein level).
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9600530 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9600530 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CRBPIII antibody
Description: RBP5 Intracellular transport of retinol. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. Note: This description may include information from UniProtKB.
Function: Intracellular transport of retinol.
Similarity: Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Function: Intracellular transport of retinol.
Similarity: Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 15 kDa
Predicted: 16 kDa
Predicted: 16 kDa
NCBI Official Full Name
retinol-binding protein 5 isoform 1
NCBI Official Synonym Full Names
retinol binding protein 5
NCBI Official Symbol
RBP5
NCBI Official Synonym Symbols
CRBP3; CRBPIII; HRBPiso; CRBP-III
NCBI Protein Information
retinol-binding protein 5
UniProt Protein Name
Retinol-binding protein 5
UniProt Gene Name
RBP5
UniProt Synonym Gene Names
CRBP-III
NCBI Description
The protein encoded by this gene is a cellular retinol-binding protein expressed highly in kidney and liver. Down-regulation of the encoded protein in hepatocellular carcinoma was associated with large tumor size and poor patient survival rates. [provided by RefSeq, Jul 2016]
Uniprot Description
Intracellular transport of retinol.
Research Articles on CRBPIII
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Product Notes
The CRBPIII rbp5 (Catalog #AAA9600530) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CRBP III Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CRBP III can be used in a range of immunoassay formats including, but not limited to, Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). IHC: 1:50-1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CRBPIII rbp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRBP III, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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