Goat Cpt1a Polyclonal Antibody | anti-Cpt1a antibody
Goat anti-Cpt1a (mouse) Antibody
Gene Names
Cpt1a; CPTI; Cpt1; C730027G07
Reactivity
Expected from sequence similarity: Mouse, Rat, Pig
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Cpt1a; Polyclonal Antibody; Goat anti-Cpt1a (mouse) Antibody; carnitine palmitoyltransferase 1A; liver; CPT1; CPT1-L; L-CPT1; C730027G07; carnitine palmitoyltransferase 1; Cpt1a antibody; liver antibody; CPT1 antibody; CPT1-L antibody; L-CPT1 antibody; C730027G07 antibody; carnitine palmitoyltransferase 1A antibody; Cpt1a (mouse); anti-Cpt1a antibody
Host
Goat
Reactivity
Expected from sequence similarity: Mouse, Rat, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PETDSHRFGKHLRQ
Sequence Length
773
Applicable Applications for anti-Cpt1a antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave next to a 90kDa band additional bands at approx.37kDa and 25kDa in Mouse and Rat Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 88.3kDa according to NP_038523.2. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Western Blot: Preliminary experiments gave next to a 90kDa band additional bands at approx.37kDa and 25kDa in Mouse and Rat Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 88.3kDa according to NP_038523.2. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Immunogen
Peptide with sequence C-PETDSHRFGKHLRQ, from the C Terminus of the protein sequence according to NP_038523.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,251 Da
NCBI Official Full Name
carnitine O-palmitoyltransferase 1, liver isoform
NCBI Official Synonym Full Names
carnitine palmitoyltransferase 1a, liver
NCBI Official Symbol
Cpt1a
NCBI Official Synonym Symbols
CPTI; Cpt1; C730027G07
NCBI Protein Information
carnitine O-palmitoyltransferase 1, liver isoform
UniProt Protein Name
Carnitine O-palmitoyltransferase 1, liver isoform
Protein Family
UniProt Gene Name
Cpt1a
UniProt Synonym Gene Names
Cpt-1; Cpt1; CPT1-L; CPT I; CPTI-L
UniProt Entry Name
CPT1A_MOUSE
Uniprot Description
CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.3.1.21; Membrane protein, multi-pass; Lipid Metabolism - fatty acid; Membrane protein, integral; Mitochondrial; Transferase
Cellular Component: integral to membrane; integral to mitochondrial outer membrane; intracellular membrane-bound organelle; membrane; mitochondrial inner membrane; mitochondrial outer membrane; mitochondrion
Molecular Function: carnitine O-palmitoyltransferase activity; identical protein binding; transferase activity; transferase activity, transferring acyl groups
Biological Process: carnitine metabolic process; eating behavior; fatty acid metabolic process; glucose metabolic process; lipid metabolic process; long-chain fatty acid metabolic process; positive regulation of fatty acid beta-oxidation; protein homooligomerization; regulation of fatty acid oxidation; regulation of insulin secretion; transport; triacylglycerol metabolic process
Research Articles on Cpt1a
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Product Notes
The Cpt1a cpt1a (Catalog #AAA422123) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Cpt1a (mouse) Antibody reacts with Expected from sequence similarity: Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Cpt1a can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Preliminary experiments gave next to a 90kDa band additional bands at approx.37kDa and 25kDa in Mouse and Rat Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 88.3kDa according to NP_038523.2. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Researchers should empirically determine the suitability of the Cpt1a cpt1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PETDSHRFGK HLRQ. It is sometimes possible for the material contained within the vial of "Cpt1a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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