Western Blot (WB)
(Western Blot analysis of CPSF3 expression in transfected 293T cell line by CPSF3 polyclonal antibody. Lane 1: CPSF3 transfected lysate (75.24kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human CPSF3 Polyclonal Antibody | anti-CPSF3 antibody
CPSF3 (Cleavage and Polyadenylation Specificity Factor Subunit 3, Cleavage and Polyadenylation Specificity Factor 73 kDa Subunit, CPSF 73 kDa Subunit, mRNA 3'-end-processing Endonuclease CPSF-73, CPSF73)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunofluorescence: 10ug/ml
Western Blot (WB)
(Western Blot analysis of CPSF3 expression in transfected 293T cell line by CPSF3 polyclonal antibody. Lane 1: CPSF3 transfected lysate (75.24kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of CPSF3 expression in transfected 293T cell line by CPSF3 polyclonal antibody. Lane 1: CPSF3 transfected lysate (75.24kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to CPSF3 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to CPSF3 on HeLa cell. [antibody concentration 10ug/ml].)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct 2012]
Uniprot Description
CPSF3: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
Protein type: EC 3.1.27.-; Ribonuclease; RNA-binding; RNA processing; RNA splicing
Chromosomal Location of Human Ortholog: 2p25.1
Cellular Component: nucleoplasm; mRNA cleavage and polyadenylation specificity factor complex; ribonucleoprotein complex
Molecular Function: 5'-3' exonuclease activity; endoribonuclease activity; protein binding; RNA binding; metal ion binding
Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; histone mRNA 3'-end processing; mRNA polyadenylation; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; mRNA cleavage
Research Articles on CPSF3
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Product Notes
The CPSF3 cpsf3 (Catalog #AAA6000125) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CPSF3 (Cleavage and Polyadenylation Specificity Factor Subunit 3, Cleavage and Polyadenylation Specificity Factor 73 kDa Subunit, CPSF 73 kDa Subunit, mRNA 3'-end-processing Endonuclease CPSF-73, CPSF73) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPSF3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the CPSF3 cpsf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSAIPAEESD QLLIRPLGAG QEVGRSCIIL EFKGRKIMLD CGIHPGLEGM DALPYIDLID PAEIDLLLIS HFHLDHCGAL PWFLQKTSFK GRTFMTHATK AIYRWLLSDY VKVSNISADD MLYTETDLEE SMDKIETINF HEVKEVAGIK FWCYHAGHVL GAAMFMIEIA GVKLLYTGDF SRQEDRHLMA AEIPNIKPDI LIIESTYGTH IHEKREEREA RFCNTVHDIV NRGGRGLIPV FALGRAQELL LILDEYWQNH PELHDIPIYY ASSLAKKCMA VYQTYVNAMN DKIRKQININ NPFVFKHISN LKSMDHFDDI GPSVVMASPG MMQSGLSREL FESWCTDKRN GVIIAGYCVE GTLAKHIMSE PEEITTMSGQ KLPLKMSVDY ISFSAHTDYQ QTSEFIRALK PPHVILVHGE QNEMARLKAA LIREYEDNDE VHIEVHNPRN TEAVTLNFRG EKLAKVMGFL ADKKPEQGQR VSGILVKRNF NYHILSPCDL SNYTDLAMST VKQTQAIPYT GPFNLLCYQL QKLTGDVEEL EIQEKPALKV FKNITVIQEP GMVVLEWLAN PSNDMYADTV TTVILEVQSN PKIRKGAVQK VSKKLEMHVY SKRLEIMLQD IFGEDCVSVK DDSILSVTVD GKTANLNLET RTVECEEGSE DDESLREMVE LAAQRLYEAL TPVH. It is sometimes possible for the material contained within the vial of "CPSF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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