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Immunofluorescence (IF) (Confocal immunofluorescent analysis of CPB1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Rabbit anti-Human CPB1 Polyclonal Antibody | anti-CPB1 antibody

CPB1 Antibody (N-term)

Gene Names
CPB1; CPB; PASP; PCPB
Reactivity
Human
Applications
Immunofluorescence, ELISA, Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CPB1; Polyclonal Antibody; CPB1 Antibody (N-term); Carboxypeptidase B; Pancreas-specific protein; PASP; CPB; PCPB; anti-CPB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CPB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human CPB1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
9-37
Sequence Length
417
Applicable Applications for anti-CPB1 antibody
Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
IF~~1:10~50
IHC~~1:10~50
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CPB1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of CPB1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(CPB1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPB1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (CPB1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPB1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(CPB1 Antibody (N-term) western blot analysis in K562,Hela cell line lysates (35ug/lane).This demonstrates the CPB1 antibody detected the CPB1 protein (arrow).)

Western Blot (WB) (CPB1 Antibody (N-term) western blot analysis in K562,Hela cell line lysates (35ug/lane).This demonstrates the CPB1 antibody detected the CPB1 protein (arrow).)

Flow Cytometry (FC/FACS)

(CPB1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (CPB1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CPB1 antibody
Three different procarboxypeptidases A and two different
procarboxypeptidases B have been isolated. The B1 and B2 forms
differ from each other mainly in isoelectric point.
Carboxypeptidase B1 is a highly tissue-specific protein and is a
useful serum marker for acute pancreatitis and dysfunction of
pancreatic transplants. It is not elevated in pancreatic carcinoma.
Product Categories/Family for anti-CPB1 antibody
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Pantoja-Uceda, D., et al. Biochemistry 47(27):7066-7078(2008)
Guimaraes, A.H., et al. Arterioscler. Thromb. Vasc. Biol. 27(10):2157-2162(2007)
Barbosa Pereira, P.J., et al. J. Mol. Biol. 321(3):537-547(2002)
Aloy, P., et al. Biol. Chem. 379(2):149-155(1998)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47368
NCBI Official Full Name
carboxypeptidase B preproprotein
NCBI Official Synonym Full Names
carboxypeptidase B1 (tissue)
NCBI Official Symbol
CPB1
NCBI Official Synonym Symbols
CPB; PASP; PCPB
NCBI Protein Information
carboxypeptidase B
UniProt Protein Name
Carboxypeptidase B
UniProt Gene Name
CPB1
UniProt Synonym Gene Names
CPB; PCPB; PASP
UniProt Entry Name
CBPB1_HUMAN

NCBI Description

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]

Uniprot Description

CPB1: Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]

Protein type: EC 3.4.17.2; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: extracellular region

Molecular Function: zinc ion binding; carboxypeptidase activity; metallocarboxypeptidase activity

Biological Process: proteolysis

Research Articles on CPB1

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Product Notes

The CPB1 cpb1 (Catalog #AAA9203154) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 9-37. The CPB1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPB1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS). IF~~1:10~50 IHC~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the CPB1 cpb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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