Rabbit anti-Human CPB1 Polyclonal Antibody | anti-CPB1 antibody

CPB1, NT (CPB1, CPB, PCPB, Carboxypeptidase B, Pancreas-specific protein) (Azide free) (HRP)

Cat. Num. AAA6288245

• Gene Names: CPB1; CPB; PASP; PCPB
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPB1; Polyclonal Antibody; NT (CPB1; CPB; PCPB; Carboxypeptidase B; Pancreas-specific protein) (Azide free) (HRP); Pancreas-specific protein; anti-CPB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CPB1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CPB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human CPB1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CPB1 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(CPB1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPB1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(CPB1 Antibody (N-term) western blot analysis in K562,Hela cell line lysates (35ug/lane).This demonstrates the CPB1 antibody detected the CPB1 protein (arrow).)

Flow Cytometry (FC/FACS)

(CPB1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CPB1 antibody

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.

Product Categories/Family for anti-CPB1 antibody

Antibodies; Enzymes; Carboxypeptidase

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Pantoja-Uceda, D., et al. Biochemistry 47(27):7066-7078(2008). Guimaraes, A.H., et al. Arterioscler. Thromb. Vasc. Biol. 27(10):2157-2162(2007). Barbosa Pereira, P.J., et al. J. Mol. Biol. 321(3):537-547(2002). Aloy, P., et al. Biol. Chem. 379(2):149-155(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 54607080
• NCBI GeneID: 1360
• NCBI Accession #: NP_001862.2
• NCBI GenBank Nucleotide #: NM_001871.2
• UniProt Accession #: P15086; O60834; Q53XJ0; Q96BQ8
• Molecular Weight: 47,368 Da
• NCBI Official Full Name: carboxypeptidase B preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase B1
• NCBI Official Symbol: CPB1
• NCBI Official Synonym Symbols: CPB; PASP; PCPB
• NCBI Protein Information: carboxypeptidase B
• UniProt Protein Name: Carboxypeptidase B
• UniProt Gene Name: CPB1
• UniProt Synonym Gene Names: CPB; PCPB; PASP
• UniProt Entry Name: CBPB1_HUMAN

NCBI Description

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]