Typical Testing Data/Standard Curve (for reference only)
Human Cornifin A (SPRR1A) ELISA Kit | SPRR1A elisa kit
Human Cornifin A (SPRR1A) ELISA Kit
Gene Names
SPRR1A; SPRK
Reactivity
Human
Synonyms
Cornifin A (SPRR1A); Human Cornifin A (SPRR1A) ELISA Kit; SPRR1A elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SPRR1A. No significant cross-reactivity or interference between SPRR1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SPRR1A and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SPRR1A elisa kit
Intended Uses: This SPRR1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanSPRR1A. This ELISA kit for research use only!
Principle of the Assay: SPRR1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SPRR1A antibody and an SPRR1A-HRP conjugate. The assay sample and buffer are incubated together with SPRR1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPRR1A concentration since SPRR1A from samples and SPRR1A-HRP conjugate compete for the anti-SPRR1A antibody binding site. Since the number of sites is limited, as more sites are occupied by SPRR1A from the sample, fewer sites are left to bind SPRR1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPRR1A concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SPRR1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SPRR1A antibody and an SPRR1A-HRP conjugate. The assay sample and buffer are incubated together with SPRR1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPRR1A concentration since SPRR1A from samples and SPRR1A-HRP conjugate compete for the anti-SPRR1A antibody binding site. Since the number of sites is limited, as more sites are occupied by SPRR1A from the sample, fewer sites are left to bind SPRR1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPRR1A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SPRR1A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9,877 Da
NCBI Official Full Name
cornifin-A
NCBI Official Synonym Full Names
small proline-rich protein 1A
NCBI Official Symbol
SPRR1A
NCBI Official Synonym Symbols
SPRK
NCBI Protein Information
cornifin-A; SPR-IA; cornifin A; 19 kDa pancornulin; small proline-rich protein IA
UniProt Protein Name
Cornifin-A
Protein Family
UniProt Gene Name
SPRR1A
UniProt Synonym Gene Names
SPR-IA
UniProt Entry Name
SPR1A_HUMAN
Uniprot Description
SPRR1A: Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Belongs to the cornifin (SPRR) family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1q21-q22
Cellular Component: cytoplasm; cornified envelope
Molecular Function: protein binding, bridging; protein binding; structural molecule activity
Biological Process: keratinocyte differentiation; epidermis development; keratinization; peptide cross-linking
Research Articles on SPRR1A
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Product Notes
The Human SPRR1A sprr1a (Catalog #AAA7244292) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7244292 ELISA Kit recognizes Human SPRR1A. It is sometimes possible for the material contained within the vial of "Cornifin A (SPRR1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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