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Western Blot (WB) (CPA3 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPA3 antibody detected the CPA3 protein (arrow).)

Rabbit anti-Human CPA3 Polyclonal Antibody | anti-CPA3 antibody

CPA3 Antibody (Center)

Gene Names
CPA3; MC-CPA
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CPA3; Polyclonal Antibody; CPA3 Antibody (Center); Mast cell carboxypeptidase A; MC-CPA; Carboxypeptidase A3; anti-CPA3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CPA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from the Central region of human CPA3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.43 (varies by lot)
Sequence Positions
255-284
Sequence Length
417
Applicable Applications for anti-CPA3 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(CPA3 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPA3 antibody detected the CPA3 protein (arrow).)

Western Blot (WB) (CPA3 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPA3 antibody detected the CPA3 protein (arrow).)

Flow Cytometry (FC/FACS)

(CPA3 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (CPA3 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CPA3 antibody
Three different forms of human pancreatic
procarboxypeptidase A have been isolated. This gene encodes a form
which is obtained as a binary complex of a procarboxypeptidase A
with proproteinase E and functions as a secretory granule
metalloexopeptidase.
Product Categories/Family for anti-CPA3 antibody
References
Pejler, G., et al. Trends Immunol. 30(8):401-408(2009)
Chen, Z.Q., et al. World J. Gastroenterol. 10(3):342-347(2004)
Huang, H., et al. Cancer Res. 59(12):2981-2988(1999)
Dikov, M.M., et al. J. Biol. Chem. 269(41):25897-25904(1994)
Natsuaki, M., et al. J. Invest. Dermatol. 99(2):138-145(1992)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48670
NCBI Official Full Name
mast cell carboxypeptidase A
NCBI Official Synonym Full Names
carboxypeptidase A3 (mast cell)
NCBI Official Symbol
CPA3
NCBI Official Synonym Symbols
MC-CPA
NCBI Protein Information
mast cell carboxypeptidase A
UniProt Protein Name
Mast cell carboxypeptidase A
UniProt Gene Name
CPA3
UniProt Synonym Gene Names
MC-CPA
UniProt Entry Name
CBPA3_HUMAN

NCBI Description

Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase. [provided by RefSeq, Jan 2009]

Uniprot Description

CPA3: Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase. [provided by RefSeq, Jan 2009]

Protein type: Protease; EC 3.4.17.1

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: transport vesicle; extracellular region; secretory granule

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: cellular protein metabolic process; angiotensin maturation; proteolysis

Research Articles on CPA3

Similar Products

Product Notes

The CPA3 cpa3 (Catalog #AAA9212217) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 255-284. The CPA3 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the CPA3 cpa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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