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Western Blot (WB) (Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS642163. This demonstrates that MBS642163 detected the CPA3 protein (arrow).)

Rabbit anti-Human CPA3 Polyclonal Antibody | anti-CPA4 antibody

CPA3, ID (CPA3, Mast cell carboxypeptidase A, Carboxypeptidase A3)

Gene Names
CPA4; CPA3
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
CPA3; Polyclonal Antibody; ID (CPA3; Mast cell carboxypeptidase A; Carboxypeptidase A3); Anti -CPA3; anti-CPA4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-CPA4 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunogen
CPA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-292 amino acids from the Central region of human CPA3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS642163. This demonstrates that MBS642163 detected the CPA3 protein (arrow).)

Western Blot (WB) (Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS642163. This demonstrates that MBS642163 detected the CPA3 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using MBS642163 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of K562 cells using MBS642163 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CPA4 antibody
Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase.
Product Categories/Family for anti-CPA4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,351 Da
NCBI Official Full Name
carboxypeptidase A4 isoform 2 preproprotein
NCBI Official Synonym Full Names
carboxypeptidase A4
NCBI Official Symbol
CPA4
NCBI Official Synonym Symbols
CPA3
NCBI Protein Information
carboxypeptidase A4; carboxypeptidase A3
UniProt Protein Name
Carboxypeptidase A4
Protein Family
UniProt Gene Name
CPA4
UniProt Synonym Gene Names
CPA3
UniProt Entry Name
CBPA4_HUMAN

NCBI Description

This gene is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7. Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. This gene could be involved in the histone hyperacetylation pathway. It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness. [provided by RefSeq, Jul 2008]

Uniprot Description

CPA4: Metalloprotease that could be involved in the histone hyperacetylation pathway. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.4.17.-; Secreted; Protease

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: extracellular region

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: histone acetylation; proteolysis

Research Articles on CPA4

Similar Products

Product Notes

The CPA4 cpa4 (Catalog #AAA642163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CPA3, ID (CPA3, Mast cell carboxypeptidase A, Carboxypeptidase A3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPA3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the CPA4 cpa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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