ELISA (EIA)
(Figure 1 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody, 9187. A sandwich ELISA was performed using immunogen or peptide (9187P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.)
Rabbit COVID 19 NSP14 Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody
Reactivity
Virus
Applications
ELISA
Purity
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is affinity chromatography purified via peptide column
Synonyms
COVID 19 NSP14 Coronavirus; Polyclonal Antibody; SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; NSP14; anti-COVID-19 antibody
Host
Rabbit
Reactivity
Virus
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is affinity chromatography purified via peptide column
Form/Format
Liquid
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is supplied in PBS containing 0.02% sodium azide.
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA)
Application Notes
Antibody validated: SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can detect 2 ng of free peptide at 1ug/mL in ELISA.
Immunogen
Anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody (9187) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP14 (ExoN) protein.
The immunogen is located between 30-80 amino acids of SARS-CoV-2 (COVID-19) NSP14 (ExoN).
The immunogen is located between 30-80 amino acids of SARS-CoV-2 (COVID-19) NSP14 (ExoN).
Homology
Predicted reactivity based on immunogen sequence: SARS-CoV NSP14 (ExoN) protein: (79%).
Conjugation
Unconjugated
Preparation and Storage
SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ELISA (EIA)
(Figure 1 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody, 9187. A sandwich ELISA was performed using immunogen or peptide (9187P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.)
ELISA (EIA)
(Figure 1 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody, 9187. A sandwich ELISA was performed using immunogen or peptide (9187P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.)
Related Product Information for anti-COVID-19 antibody
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP14 (ExoN) is an enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity (3,4,5). It acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens(6,7,8).
Product Categories/Family for anti-COVID-19 antibody
References
Gorbalenya. bioRxiv: 2020.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Minskaia et. al. Proc. Natl. Acad. Sci. U.S.A 2006; 103:5108-5113
Bouvet et. al. PLoS Pathog. 2010; 6:E1000863-E1000863
Bouvet et. al. Proc. Natl. Acad. Sci. U.S.A. 2012;109:9372-9377
Denison et. al. RNA Biol. 2011;8:270-279
Smith et. al. PLoS Pathog. 2013;9:e1003565-e1003565
Agostini et. al. MBio 2018; 9:0-0
Hui et al. Int J Infect Dis. 2020;91:264-266.
Minskaia et. al. Proc. Natl. Acad. Sci. U.S.A 2006; 103:5108-5113
Bouvet et. al. PLoS Pathog. 2010; 6:E1000863-E1000863
Bouvet et. al. Proc. Natl. Acad. Sci. U.S.A. 2012;109:9372-9377
Denison et. al. RNA Biol. 2011;8:270-279
Smith et. al. PLoS Pathog. 2013;9:e1003565-e1003565
Agostini et. al. MBio 2018; 9:0-0
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular Mass: 62.9 kDa
Actual Protein Molecular Mass: 60 kDa
Actual Protein Molecular Mass: 60 kDa
NCBI Official Full Name
3'-to-5' exonuclease
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Product Notes
The COVID-19 (Catalog #AAA154676) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 NSP14 Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Antibody validated: SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can detect 2 ng of free peptide at 1ug/mL in ELISA. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 NSP14 Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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