Rabbit anti-Human, Mouse Cortactin Polyclonal Antibody | anti-CTTN antibody

Cortactin, phosphorylated (Tyr466) (CTTN)

Cat. Num. AAA623923

• Gene Names: CTTN; EMS1; FLJ34459
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Cortactin; Polyclonal Antibody; phosphorylated (Tyr466) (CTTN); Anti -Cortactin; anti-CTTN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Cortactin when phosphorylated at tyrosine 466. Species Cross-Reactivity: human.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-CTTN antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot:1:500-1:1,000
• Immunogen: Synthetic phosphopeptide corresponding to mouse Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CTTN antibody

Cortactin is an 80/85kD cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37aa sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an a-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks.

Product Categories/Family for anti-CTTN antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 40645044
• NCBI GeneID: 2017
• UniProt Accession #: Q76MU0; Q14247; Q53HG7; Q8N707; Q96H99
• Molecular Weight: 35,688 Da
• NCBI Official Full Name: cortactin
• NCBI Official Synonym Full Names: cortactin
• NCBI Official Symbol: CTTN
• NCBI Official Synonym Symbols: EMS1; FLJ34459
• NCBI Protein Information: src substrate cortactin; amplaxin; 1110020L01Rik; oncogene EMS1; OTTHUMP00000165961; OTTHUMP00000165962; OTTHUMP00000235667; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)
• UniProt Protein Name: Cortactin
• Protein Family: Cortactin-binding protein
• UniProt Gene Name: EMS1
• UniProt Entry Name: Q76MU0_HUMAN

NCBI Description

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Uniprot Description

Cortactin: a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin may play a role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. It is degraded in a caspase-dependent manner during apoptosis. It is overexpressed in numerous cancers with poor patient prognosis. It may contribute to tumor cell invasion and metastasis. Two splice variant isoforms have been identified.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cortical cytoskeleton; ruffle; voltage-gated potassium channel complex; cytoskeleton; focal adhesion; growth cone; lamellipodium; cytoplasm; dendritic spine; podosome; coated pit; actin filament; cell cortex

Molecular Function: protein binding

Biological Process: focal adhesion formation; receptor-mediated endocytosis; intracellular protein transport; positive regulation of actin filament polymerization; actin filament polymerization; actin cytoskeleton reorganization; cell motility involved in cell locomotion; regulation of axon extension; substrate-bound cell migration, cell extension; neurite morphogenesis