Western Blot (WB)
(Western blot analysis of extracts from Hela cells, untreated or treated with UV (20min), using Cortactin antibody and Cortactin antibody (Tyr466) (Lanes 3 and 4))
Rabbit Cortactin Polyclonal Antibody | anti-CCTN antibody
Cortactin Antibody (Tyr466)
Gene Names
Cortactin; CG3637; cortactin; cttn; DmelCG3637
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
Cortactin; Polyclonal Antibody; Cortactin Antibody (Tyr466); Rabbit Polyclonal Cortactin Antibody (Tyr466); Polyclonal Cortactin antibody; Anti-Cortactin antibody; 1110020L01Rik antibody; Amplaxin antibody; cortactin antibody; CTTN antibody; EMS1 antibody; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate) antibody; FLJ34459 antibody; Oncogene EMS1 antibody; Src substrate cortactin antibody; SRC8 antibody; anti-CCTN antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Cortactin antibody (Tyr466) detects endogenous levels of Cortactin only when phosphorylated at tyrosine 466
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
559
Applicable Applications for anti-CCTN antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Cortactin antibody was raised in Rabbit using a synthesized phosphopeptide derived from mouse Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T) as the immunogen.
Cross-Reactivity
Mouse
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, untreated or treated with UV (20min), using Cortactin antibody and Cortactin antibody (Tyr466) (Lanes 3 and 4))
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, untreated or treated with UV (20min), using Cortactin antibody and Cortactin antibody (Tyr466) (Lanes 3 and 4))
Related Product Information for anti-CCTN antibody
Inactive cortactin diffuses throughout the cytoplasm, but upon phosphorylation, the protein begins to target certain areas in the cell. Cortactin-assisted Arp2/3-nucleated actin branches are most prominent in the actin cortex, around the periphery of the cell. A phosphorylated cortactin monomer binds to, activates, and stabilizes an Arp2/3 complex on preexisting F-actin, which provides a nucleation site for a new actin branch to form from the "mother" filament. Branches formed from cortactin-assisted nucleation sites are very stable; cortactin has been shown to inhibit debranching. Thus, polymerization and branching of actin is promoted in areas of the cell where cortactin is localized.
Product Categories/Family for anti-CCTN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cortactin, isoform D
NCBI Official Symbol
Cortactin
NCBI Official Synonym Symbols
CG3637; cortactin; cttn; DmelCG3637
NCBI Protein Information
cortactin
Protein Family
Similar Products
Product Notes
The CCTN (Catalog #AAA5313687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cortactin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the CCTN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cortactin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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