Rabbit Cortactin Polyclonal Antibody | anti-CTTN antibody

Cortactin

Cat. Num. AAA616278

• Gene Names: CTTN; EMS1; FLJ34459
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: Cortactin; Polyclonal Antibody; Anti -Cortactin; anti-CTTN antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human total cortactin protein.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-CTTN antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry (Paraffin): 1:50; Optimal dilution determined by the researcher.
• Immunogen: Synthetic peptide corresponding to the C-terminal residues of human cortactin (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from C6, COS and HeLa cells untreated and treated with hydrogen peroxide (H2O2), using MBS616278.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of cortactin in paraffin-embedded human breast carcinoma showing cytoplasmic and membrane localization, using MBS616278.)

Related Product Information for anti-CTTN antibody

Cortactin is a cortical actin binding protein. Its N-terminal acidic domain (NTA) associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acid that are important in F-actin binding and cross-linking. The C-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin is involved in signaling events that coordinate actin reorganization during cell movement. The human cortactin homologue EMS1 is overexpressed in numerous cancers with poor patient prognosis. Cortactin may also play an important role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network.

Product Categories/Family for anti-CTTN antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 40645044
• NCBI GeneID: 2017
• UniProt Accession #: Q76MU0; Q14247; Q53HG7; Q8N707; Q96H99
• Molecular Weight: 35,688 Da
• NCBI Official Full Name: cortactin
• NCBI Official Synonym Full Names: cortactin
• NCBI Official Symbol: CTTN
• NCBI Official Synonym Symbols: EMS1; FLJ34459
• NCBI Protein Information: src substrate cortactin; amplaxin; 1110020L01Rik; oncogene EMS1; OTTHUMP00000165961; OTTHUMP00000165962; OTTHUMP00000235667; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)
• UniProt Protein Name: Cortactin
• Protein Family: Cortactin-binding protein
• UniProt Gene Name: EMS1
• UniProt Entry Name: Q76MU0_HUMAN

NCBI Description

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Uniprot Description

Cortactin: a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin may play a role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. It is degraded in a caspase-dependent manner during apoptosis. It is overexpressed in numerous cancers with poor patient prognosis. It may contribute to tumor cell invasion and metastasis. Two splice variant isoforms have been identified.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cortical cytoskeleton; ruffle; voltage-gated potassium channel complex; focal adhesion; cytoskeleton; growth cone; lamellipodium; cytoplasm; dendritic spine; coated pit; podosome; actin filament; cell cortex

Molecular Function: protein binding

Biological Process: focal adhesion formation; receptor-mediated endocytosis; intracellular protein transport; positive regulation of actin filament polymerization; actin filament polymerization; actin cytoskeleton reorganization; cell motility involved in cell locomotion; regulation of axon extension; substrate-bound cell migration, cell extension; neurite morphogenesis