Rabbit anti-Human UNC84B Polyclonal Antibody | anti-UNC84B antibody

UNC84B, ID (SUN2, FRIGG, KIAA0668, RAB5IP, UNC84B, SUN domain-containing protein 2, Protein unc-84 homolog B, Rab5-interacting protein, Sad1/unc-84 protein-like 2) (Azide free) (HRP)

Cat. Num. AAA6361513

• Gene Names: SUN2; UNC84B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UNC84B; Polyclonal Antibody; ID (SUN2; FRIGG; KIAA0668; RAB5IP; SUN domain-containing protein 2; Protein unc-84 homolog B; Rab5-interacting protein; Sad1/unc-84 protein-like 2) (Azide free) (HRP); SUN2; Sad1/unc-84 protein-like 2; anti-UNC84B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-UNC84B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UNC84B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-265 amino acids from the Central region of human UNC84B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UNC84B Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the UNC84B antibody detected the UNC84B protein (arrow).)

Related Product Information for anti-UNC84B antibody

SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).[supplied by OMIM].

Product Categories/Family for anti-UNC84B antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Turgay, Y., et al. EMBO J. 29(14):2262-2275(2010). Haque, F., et al. J. Biol. Chem. 285(5):3487-3498(2010). Stewart-Hutchinson, P.J., et al. Exp. Cell Res. 314(8):1892-1905(2008). Cerhan, J.R., et al. Blood 110(13):4455-4463(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 31742498
• NCBI GeneID: 25777
• NCBI Accession #: NP_056189.1
• NCBI GenBank Nucleotide #: NM_015374.2
• UniProt Accession #: Q9UH99; O75156; Q2NKN8; Q2T9F7; Q504T5; Q6B4H1; Q7Z3E3; B0QY62
• Molecular Weight: 79,612 Da
• NCBI Official Full Name: SUN domain-containing protein 2 isoform b
• NCBI Official Synonym Full Names: Sad1 and UNC84 domain containing 2
• NCBI Official Symbol: SUN2
• NCBI Official Synonym Symbols: UNC84B
• NCBI Protein Information: SUN domain-containing protein 2
• UniProt Protein Name: SUN domain-containing protein 2
• UniProt Gene Name: SUN2
• UniProt Synonym Gene Names: FRIGG; KIAA0668; RAB5IP; UNC84B; Rab5IP
• UniProt Entry Name: SUN2_HUMAN

NCBI Description

SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).[supplied by OMIM, Nov 2010]

Uniprot Description

SUN2: a nuclear transmembrane protein. Forms part of a membrane-spanning fibrillar complex that interconnects attached meiotic telomeres with cytoplasmic structures. Together with SUN1 connects the nuclear envelope with the centrosome. Interacts with RAB5A. Reportedly expressed in the endosome where it may play a role in endosome fusion and endocytosis.

Protein type: Membrane protein, integral; Nuclear envelope

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nuclear membrane; condensed nuclear chromosome; nuclear chromosome, telomeric region; endosome membrane; integral to nuclear inner membrane; nuclear envelope

Molecular Function: identical protein binding; protein binding; microtubule binding; lamin binding

Biological Process: mitotic spindle organization and biogenesis; centrosome localization; nuclear migration along microfilament; nuclear migration; nuclear membrane organization and biogenesis; positive regulation of cell migration