Rabbit anti-Human, Mouse COPZ2 Polyclonal Antibody | anti-COPZ2 antibody

COPZ2, CT (COPZ2, Coatomer subunit zeta-2, Zeta-2-coat protein)

Cat. Num. AAA6010007

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A affinity chromatography.
• Synonyms: COPZ2; Polyclonal Antibody; CT (COPZ2; Coatomer subunit zeta-2; Zeta-2-coat protein); Anti -COPZ2; anti-COPZ2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COPZ2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-COPZ2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Western Blot: 1:1000; Immunohistochemistry (FFPE): 1:50-100; Flow Cytometry: 1:10-50; ; Optimal dilutions to be determined by the researcher.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 156-184 in the C-teminal region of human COPZ
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse spleen tissue lysates (35ug/lane) using MBS6010007. COPZ2 (arrow) was detected using MBS6010007.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using MBS6010007 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MBS6010007 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Indirect Flow cytometric analysis of U251 cells (right histogram) using MBS6010007 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-COPZ2 antibody

COPZ2 encodes a subunit of the coatomer protein complex, a seven-subunit complex that functions in the formation of COPI-type, non-clathrin-coated vesicles. COPI vesicles function in the retrograde Golgi-to-ER transport of dilysine-tagged proteins. COPZ2 is similar to a related family member, and the two encoded proteins form distinct isotypes of the coatomer protein complex.

Product Categories/Family for anti-COPZ2 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7705983
• NCBI GeneID: 51226
• NCBI Accession #: NP_057513.1
• NCBI GenBank Nucleotide #: NM_016429.2
• UniProt Accession #: Q9P299
• NCBI Official Full Name: coatomer subunit zeta-2
• NCBI Official Synonym Full Names: coatomer protein complex, subunit zeta 2
• NCBI Official Symbol: COPZ2
• NCBI Protein Information: coatomer subunit zeta-2; zeta2-COP; zeta-2 COP; zeta-2 coat protein; zeta-2-coat protein; nonclathrin coat protein zeta-COP
• UniProt Protein Name: Coatomer subunit zeta-2
• Protein Family: Coatomer
• UniProt Gene Name: COPZ2
• UniProt Synonym Gene Names: Zeta-2 COP
• UniProt Entry Name: COPZ2_HUMAN

NCBI Description

This gene encodes a subunit of the coatomer protein complex, a seven-subunit complex that functions in the formation of COPI-type, non-clathrin-coated vesicles. COPI vesicles function in the retrograde Golgi-to-ER transport of dilysine-tagged proteins. This gene is similar to a related family member, and the two encoded proteins form distinct isotypes of the coatomer protein complex. [provided by RefSeq, Jul 2008]

Uniprot Description

COPZ2: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex. Belongs to the adaptor complexes small subunit family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: ER-Golgi intermediate compartment membrane; cis-Golgi network; COPI vesicle coat

Biological Process: vesicle-mediated transport; intracellular protein transport