Immunoprecipitation (IP)
(rat COPZ1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate4 uL anti-rat COPZ1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat COPZ1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)
Rabbit anti-Rat COPZ1 Polyclonal Antibody | anti-COPZ1 antibody
Anti-COPZ1 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Immunoprecipitation (IP)
(rat COPZ1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate4 uL anti-rat COPZ1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat COPZ1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)
Immunoprecipitation (IP)
(rat COPZ1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate4 uL anti-rat COPZ1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat COPZ1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)
Western Blot (WB)
(Anti-COPZ1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: COLO205 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:20 kDaObserved band size:18 kDa)
Western Blot (WB)
(Anti-COPZ1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: COLO205 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:20 kDaObserved band size:18 kDa)
NCBI and Uniprot Product Information
Uniprot Description
COPZ1: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the adaptor complexes small subunit family.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q13.2-q13.3
Cellular Component: COPI vesicle coat; cytosol
Biological Process: intracellular protein transport; intra-Golgi vesicle-mediated transport; COPI coating of Golgi vesicle; retrograde vesicle-mediated transport, Golgi to ER
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Product Notes
The COPZ1 copz1 (Catalog #AAA8113065) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-COPZ1 Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's COPZ1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the COPZ1 copz1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COPZ1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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