Rabbit anti-Human, Mouse COPZ1 Polyclonal Antibody | anti-COPZ1 antibody

COPZ1, NT (COPZ1, COPZ, Coatomer subunit zeta-1, Zeta-1-coat protein) (Biotin)

Cat. Num. AAA6287913

• Gene Names: COPZ1; COPZ; CGI-120; HSPC181; zeta-COP; zeta1-COP
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COPZ1; Polyclonal Antibody; NT (COPZ1; COPZ; Coatomer subunit zeta-1; Zeta-1-coat protein) (Biotin); Zeta-1-coat protein; anti-COPZ1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-COPZ1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: COPZ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-53 amino acids from the N-terminal region of human COPZ1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(COPZ1 Antibody (N-term) western blot analysis in L929 cell line lysates (35ug/lane).This demonstrates the COPZ1 antibody detected the COPZ1 protein (arrow).)

Western Blot (WB)

(COPZ1 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the COPZ1 antibody detected the COPZ1 protein (arrow).)

Related Product Information for anti-COPZ1 antibody

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.

Product Categories/Family for anti-COPZ1 antibody

Antibodies; Proteins

References

Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Lippincott-Schwartz, J., et al. Trends Cell Biol. 16 (10), E1-E4 (2006): Wegmann, D., et al. Mol. Cell. Biol. 24(3):1070-1080(2004). Futatsumori, M., et al. J. Biochem. 128(5):793-801(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 7706337
• NCBI GeneID: 22818
• NCBI Accession #: NP_057141.1
• NCBI GenBank Nucleotide #: NM_016057.1
• UniProt Accession #: P61923; Q549N6; Q9Y3C3; B4DDX8; B4DHZ0; F8VS17; F8VWL5
• Molecular Weight: 22.3 kDa (197aa) confirmed by MALDI-TOF
• NCBI Official Full Name: coatomer subunit zeta-1 isoform 1
• NCBI Official Synonym Full Names: coatomer protein complex, subunit zeta 1
• NCBI Official Symbol: COPZ1
• NCBI Official Synonym Symbols: COPZ; CGI-120; HSPC181; zeta-COP; zeta1-COP
• NCBI Protein Information: coatomer subunit zeta-1
• UniProt Protein Name: Coatomer subunit zeta-1
• Protein Family: Coatomer
• UniProt Gene Name: COPZ1
• UniProt Synonym Gene Names: COPZ; Zeta-1 COP
• UniProt Entry Name: COPZ1_HUMAN

Uniprot Description

COPZ1: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the adaptor complexes small subunit family.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13.2-q13.3

Cellular Component: COPI vesicle coat; cytosol

Biological Process: intracellular protein transport; intra-Golgi vesicle-mediated transport; COPI coating of Golgi vesicle; retrograde vesicle-mediated transport, Golgi to ER