Immunohistochemistry (IHC)
(Immunochemical staining of rat CNTN3 in rat brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Rat Contactin 3 Polyclonal Antibody | anti-CNTN3 antibody
Anti-Contactin 3 Antibody, Rabbit Polyclonal
Gene Names
Cntn3; Pang; BIG-1
Reactivity
Rat
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Contactin 3; Polyclonal Antibody; Anti-Contactin 3 Antibody; Rabbit Polyclonal; Contactin 3/CNTN3 Antibody; Rabbit PAb; Antigen Affinity Purified; contactin 3 (plasmacytoma associated); anti-CNTN3 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rat Contactin 3/CNTN3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CNTN3 antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IHC-P: 1:500-1:2000
Immunogen
Recombinant Rat Contactin 3/CNTN3 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Rat Contactin 3/CNTN3. Contactin 3/CNTN3 specific IgG was purified by Rat Contactin 3/CNTN3 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of rat CNTN3 in rat brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat CNTN3 in rat brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-CNTN3 antibody
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2 (TAG-1), Contactin-3 (BIG-1), BIG-2, Contactin-5 (NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-3, also known as CNTN3 (BIG-1 in rat and PANG in mouse), is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. In the brain, Contactin-3 is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3. The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.
References
Yoshihara Y, et al. (1994) BIG-1: a new TAG-1/F3-related member of the immunoglobulin superfamily with neurite outgrowth-promoting activity. Neuron 13(2):415-26.Yoshihara Y, et al. (1995) Overlapping and differential expression of BIG-2, BIG-1, TAG-1, and F3: four members of an axon-associated cell adhesion molecule subgroup of the immunoglobulin superfamily. Journal of neurobiology 28(1):51-69.Yoshihara Y, et al. (1995) Overlapping and differential expression of BIG-2, BIG-1, TAG-1, and F3: four members of an axon-associated cell adhesion molecule subgroup of the immunoglobulin superfamily. Journal of neurobiology 28(1):51-69.Shimoda Y, et al. (2009) Contactins: Emerging key roles in the development and function of the nervous system. Cell adhesion & migration 3(1):64-70.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
112,789 Da
NCBI Official Full Name
contactin-3
NCBI Official Synonym Full Names
contactin 3 (plasmacytoma associated)
NCBI Official Symbol
Cntn3
NCBI Official Synonym Symbols
Pang; BIG-1
NCBI Protein Information
contactin-3; brain-derived immunoglobulin superfamily protein 1; Plasmacytoma-associated neuronal glycoprotein (neural cell adhesion molecule BIG-1)
UniProt Protein Name
Contactin-3
Protein Family
UniProt Gene Name
Cntn3
UniProt Synonym Gene Names
BIG-1
UniProt Entry Name
CNTN3_RAT
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Product Notes
The CNTN3 cntn3 (Catalog #AAA8112936) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Contactin 3 Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Contactin 3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the CNTN3 cntn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Contactin 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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