Contactin 3 Recombinant Protein | CNTN3 recombinant protein

Contactin 3 Protein, Human, Recombinant (708 Asp/Asn, His Tag)

Cat. Num. AAA8120054

• Gene Names: CNTN3; PCS; PANG; BIG-1
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Contactin 3; Contactin 3 Protein; Human; Recombinant (708 Asp/Asn; His Tag); Human Contactin 3/CNTN3 Protein (708 Asp/Asn; BIG-1 Protein; PANG Protein; PCS Protein; contactin 3 (plasmacytoma associated); CNTN3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser1002, 708Asp/Asn

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 20
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CNTN3 (NP_065923.1)(Met 1-Ser1002, 708 Asp/Asn) precursor was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CNTN3 recombinant protein

Background: Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2 (TAG-1), Contactin-3 (BIG-1), BIG-2, Contactin-5 (NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-3, also known as CNTN3 (BIG-1 in rat and PANG in mouse), is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. In the brain, Contactin-3 is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3. The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.

References

Yoshihara Y, et al. (1994) BIG-1: a new TAG-1/F3-related member of the immunoglobulin superfamily with neurite outgrowth-promoting activity. Neuron 13(2):415-26.Yoshihara Y, et al. (1995) Overlapping and differential expression of BIG-2, BIG-1, TAG-1, and F3: four members of an axon-associated cell adhesion molecule subgroup of the immunoglobulin superfamily. Journal of neurobiology 28(1):51-69.Yoshihara Y, et al. (1995) Overlapping and differential expression of BIG-2, BIG-1, TAG-1, and F3: four members of an axon-associated cell adhesion molecule subgroup of the immunoglobulin superfamily. Journal of neurobiology 28(1):51-69.Shimoda Y, et al. (2009) Contactins: Emerging key roles in the development and function of the nervous system. Cell adhesion & migration 3(1):64-70.

NCBI and Uniprot Product Information

• NCBI GI #: 75709184
• NCBI GeneID: 5067
• NCBI Accession #: NP_065923.1
• NCBI GenBank Nucleotide #: NM_020872.1
• UniProt Accession #: Q9P232; Q9H039; B9EK50
• Molecular Weight: 112,883 Da
• NCBI Official Full Name: contactin-3
• NCBI Official Synonym Full Names: contactin 3 (plasmacytoma associated)
• NCBI Official Symbol: CNTN3
• NCBI Official Synonym Symbols: PCS; PANG; BIG-1
• NCBI Protein Information: contactin-3; plasmacytoma-associated neuronal glycoprotein; brain-derived immunoglobulin superfamily protein 1
• UniProt Protein Name: Contactin-3
• Protein Family: Contactin
• UniProt Gene Name: CNTN3
• UniProt Synonym Gene Names: KIAA1496; PANG; BIG-1
• UniProt Entry Name: CNTN3_HUMAN

Uniprot Description

CNTN3: Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. Belongs to the immunoglobulin superfamily. Contactin family.

Protein type: Cell surface; Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3p12.3

Cellular Component: plasma membrane

Biological Process: nervous system development; cell adhesion