SDS-PAGE
GTP cyclohydrolase-2 Recombinant Protein | GTP recombinant protein
Recombinant E Coli GTP cyclohydrolase-2
Gene Names
ribA; ECK1272; JW1269
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
GTP cyclohydrolase-2; Recombinant E Coli GTP cyclohydrolase-2; GTP cyclohydrolase II; GTP recombinant protein
Host
E Coli
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid containing glycerol
Sequence Positions
1-196aa; Full Length
Sequence
MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHTPVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKIRAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTEAGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK
Sequence Length
196
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for GTP recombinant protein
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.
References
Biosynthesis of riboflavin
cloning, sequencing, mapping, and expression of the gene coding for GTP cyclohydrolase II in Escherichia coli.Richter G., Ritz H., Katzenmeier G., Volk R., Kohnle A., Lottspeich F., Allendorf D., Bacher A.J. Bacteriol. 175:4045-4051(1993)
A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.DNA Res. 3:363-377(1996)
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997)
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)
The aconitase of Escherichia coli. Nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerases.Prodromou C., Artymiuk P.J., Guest J.R.Eur. J. Biochem. 204:599-609(1992)
Purification and properties of guanosine triphosphate cyclohydrolase II from Escherichia coli.Foor F., Brown G.M.J. Biol. Chem. 250:3545-3551(1975)
Biosynthesis of riboflavin
studies on the mechanism of GTP cyclohydrolase II.Ritz H., Schramek N., Bracher A., Herz S., Eisenreich W., Richter G., Bacher A.J. Biol. Chem. 276:22273-22277(2001)
Biosynthesis of vitamin B2.Kaiser J., Schramek N., Eberhardt S., Puettmer S., Schuster M., Bacher A.Eur. J. Biochem. 269:5264-5270(2002)
GTP cyclohydrolase II structure and mechanism.Ren J., Kotaka M., Lockyer M., Lamb H.K., Hawkins A.R., Stammers D.K.J. Biol. Chem. 280:36912-36919(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.8 kDa
NCBI Official Full Name
GTP cyclohydrolase II
NCBI Official Symbol
ribA
NCBI Official Synonym Symbols
ECK1272; JW1269
NCBI Protein Information
GTP cyclohydrolase II
UniProt Protein Name
GTP cyclohydrolase-2
Protein Family
UniProt Gene Name
ribA
UniProt Entry Name
RIBA_ECOLI
NCBI Description
GTP cyclohydrolase II catalyzes the opening of the imidazole ring of GTP and removal of pyrophosphate, the first committed step in riboflavin biosynthesis. [More information is available at EcoCyc: EG11331].
Uniprot Description
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.
Research Articles on GTP
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Product Notes
The GTP riba (Catalog #AAA1265364) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-196aa; Full Length. The amino acid sequence is listed below: MQLKRVAEAK LPTPWGDFLM VGFEELATGH DHVALVYGDI SGHTPVLARV HSECLTGDAL FSLRCDCGFQ LEAALTQIAE EGRGILLYHR QEGRNIGLLN KIRAYALQDQ GYDTVEANHQ LGFAADERDF TLCADMFKLL GVNEVRLLTN NPKKVEILTE AGINIVERVP LIVGRNPNNE HYLDTKAEKM GHLLNK. It is sometimes possible for the material contained within the vial of "GTP cyclohydrolase-2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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