Rabbit anti-Mouse Connexin 40 Polyclonal Antibody | anti-CX40 antibody
Connexin 40, CT (CX40, Gap Junction Alpha 5 Protein, CXA-5) (Biotin)
Reactivity
Mouse
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Connexin 40; Polyclonal Antibody; CT (CX40; Gap Junction Alpha 5 Protein; CXA-5) (Biotin); anti-CX40 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Connexin 40 at ~40kD. Reactivity has been confirmed with mouse heart homogenates by Western Blot, mouse heart and lung homogenates by immunoprecipitation, and mouse heart tissue by immunohistochemistry and immunofluorescence.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Applicable Applications for anti-CX40 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Frozen, Immunoprecipitation (IP)
Application Notes
IF: 1-3ug/ml
WB: 1-3ug/ml
IHC-F: 1-3ug/ml; Mice were perfused transcardially with 3ml pre-fixative (50mM phosphate buffer, 0.1% sodium nitrite, 1 unit/ml heparin), followed by 40ml fixative (0.16M sodium phosphate buffer, 0.2% picric acid, 1-, 2-, or 4% paraformaldehyde) and perfusion with 10ml sucrose wash (10% sucrose in 25mM sodium phosphate buffer, pH 7.4). Tissues were then stored in cryoprotectant (10% sucrose, 25mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.
IP: 7ug/reaction
Applications are based on unconjugated antibody.
WB: 1-3ug/ml
IHC-F: 1-3ug/ml; Mice were perfused transcardially with 3ml pre-fixative (50mM phosphate buffer, 0.1% sodium nitrite, 1 unit/ml heparin), followed by 40ml fixative (0.16M sodium phosphate buffer, 0.2% picric acid, 1-, 2-, or 4% paraformaldehyde) and perfusion with 10ml sucrose wash (10% sucrose in 25mM sodium phosphate buffer, pH 7.4). Tissues were then stored in cryoprotectant (10% sucrose, 25mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.
IP: 7ug/reaction
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to the C-terminal region of mouse and rat Connexin 40 (CX40) proteins. The human sequence at this location differs by only one amino acid. Species sequence homology: rat and human
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Categories/Family for anti-CX40 antibody
References
1. Beyer, E.C., et al., J. Membr. Biol. 127: 69-76 (1992). 2. Bruzzone, R., et al., Mol. Biol. Cell 4: 7-20 (1993). 3. Hennemann, H., et al., J. Cell. Biol. 117: 1299-1310 (1992). 4. Coppen, S.R., et al., Dev. Genet. 24: 82-90 (1999). 5. van Kempen, M.J., et al., Cardiovasc. Res. 32: 886-900 (1996). 6. Verheule, S., et al., Circ. Res. 80: 673-681 (1997). 7. Darrow, B.J., et al., Circ. Res. 76: 381-387 (1995). 8. Verheule, S., et al., J. Cardiovasc. Electrophysiol. 10: 1380-1389 (1999). 9. Tamaddon, H.S., et al., Circ. Res. 87: 929-936 (2000). 10. van Rijen, H.V., et al., Circulation 103: 1591-1598 (2001). 11. van Kempen, M.J. et al., Histochem. Cell. Biol. 112: 479-486 (1999). 12. Vozzi, C., et al., J. Mol. Cell. Cardiol. 31: 991-1003 (1999). 13. Yeh, H.I., et al., Arterioscler. Thromb. Vasc. Biol. 20: 1753-1762 (2000). 14. Cottrell, G.T. et al., Am. J. Physiol. Cell. Physiol. 281: C1559-C1567 (2001). 15. He, D.S., et al., PNAS 96: 6495-6500 (1999). 16. Valiunas, V., et al., Am. J. Physiol. Heart Circ. Physiol. 281: H1675-H1689 (2001). 17. Elenes, S., et al., J. Cardiovasc. Electrophysiol. 10: 990-1004 (1999).
Similar Products
Product Notes
The CX40 (Catalog #AAA6470158) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Connexin 40, CT (CX40, Gap Junction Alpha 5 Protein, CXA-5) (Biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 40 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Frozen, Immunoprecipitation (IP). IF: 1-3ug/ml WB: 1-3ug/ml IHC-F: 1-3ug/ml; Mice were perfused transcardially with 3ml pre-fixative (50mM phosphate buffer, 0.1% sodium nitrite, 1 unit/ml heparin), followed by 40ml fixative (0.16M sodium phosphate buffer, 0.2% picric acid, 1-, 2-, or 4% paraformaldehyde) and perfusion with 10ml sucrose wash (10% sucrose in 25mM sodium phosphate buffer, pH 7.4). Tissues were then stored in cryoprotectant (10% sucrose, 25mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning. IP: 7ug/reaction Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CX40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
